Flow-through pore characteristics of monolithic silicas and their impact on column performance in high-performance liquid chromatography.
Skudas R, Grimes BA, Thommes M, Unger KK (2009)
Publication Type: Journal article, Original article
Subtype: other
Publication year: 2009
Journal
Publisher: Elsevier B.V.
Book Volume: 1216
Pages Range: 2625-2636-2636
Journal Issue: 13
DOI: 10.1016/j.chroma.2009.01.079
Abstract
To elucidate the role of the flow-through characteristics with regard to the column performance in HPLC native and n-octadecyl bonded monolithic silica rods and columns, resp. of 100 mm length and 4.6 mm ID with mesopores at 10-25 nm and macropores at 0.7-6.0 μm were examd. by mercury intrusion/extrusion, SEM, image anal. and permeability. The obtained data of the flow-through pore sizes and porosity values as well as surface-to-vol. ratio of the stationary phase skeleton enabled to predict their influence to the chromatog. sepn. efficiency. The authors' data demonstrate that mercury porosimetry is a reliable technique to obtain all the characteristic parameters of the flow-through pores of silica monoliths. An important result of the authors' examn. was that the surface-to-vol. ratio of monolithic silica skeletons had more significant impact to the sepn. process, rather than the av. flow-through pore sizes. The authors could also show the essential differences between the particulate and monolithic stationary phases based on theor. computation. The results, obtained from other characterization methods also indicated the structural complexity of monolithic silica samples. Permeability of columns is a generally applicable parameter to characterize all chromatog. phases no matter the chem. or format. The correlation coeff. obtained for mercury intrusion and permeability of water was 0.998, though the authors' study revealed that the surface modification is more likely influencing the obtained results. Further, the assumption of the cylindrical morphol. of flow-through pores is not relevant to the studied monolithic silica columns. These results on the morphol. of the flow-through pores and of the skeletons were confirmed by the image anal. as well. The authors' main finding is that the flow-through pore sizes are not relevant for the estn. of the chromatog. sepn. efficiency of monolithic silica columns. [on SciFinder(R)]
Authors with CRIS profile
Involved external institutions
Johannes Gutenberg-Universität Mainz (JGU)
Germany (DE)
NTNU Trondheim - Norwegian University of Science and Technology
Norway (NO)
Quantachrome Instruments
United States (USA) (US)
Germany (DE)
NTNU Trondheim - Norwegian University of Science and Technology
Norway (NO)
Quantachrome Instruments
United States (USA) (US)
How to cite
APA:
Skudas, R., Grimes, B.A., Thommes, M., & Unger, K.K. (2009). Flow-through pore characteristics of monolithic silicas and their impact on column performance in high-performance liquid chromatography. Journal of Chromatography A, 1216(13), 2625-2636-2636. https://dx.doi.org/10.1016/j.chroma.2009.01.079
MLA:
Skudas, R., et al. "Flow-through pore characteristics of monolithic silicas and their impact on column performance in high-performance liquid chromatography." Journal of Chromatography A 1216.13 (2009): 2625-2636-2636.
BibTeX: Download