Influence of the hydrophilic-hydrophobic contrast of porous surfaces on the enzymatic performance.

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autorinnen und Autoren: Fried DI, Bednarski D, Dreifke M, Brieler FJ, Thommes M, Froba M
Zeitschrift: Journal of Materials Chemistry B
Verlag: Royal Society of Chemistry
Jahr der Veröffentlichung: 2015
Band: 3
Heftnummer: 11
Seitenbereich: 2341-2349-2349
ISSN: 2050-7518


Abstract

Herein we report the dependency of the performance (uptake, activity, stability) of hydrophilic glucose-6-phosphate dehydrogenase (G6PDH) onto mesoporous cellular foams (MCF) grafted with aminosilanes of different chain length. The resulting hydrophobic-hydrophilic contrast was carefully evaluated by combined argon and water sorption and quantified with a newly developed hydrophilicity index suitable for the characterization of large-pore materials. The enzymic behavior was influenced by electrostatic and hydrophobic interactions between both reaction partners due to the creation of specific microenvironments inside the pore system. The microenvironment created by the materials with high hydrophobicity did not contribute to the beneficial electrostatic interactions of the charged amine groups by increased hydrophobic forces instead a competition of both forces was present. Thus an improved biocatalytic performance was not obsd. for materials with high hydrophobicity but short-chain functionalized MCFs led to highly stable and active biocatalysts. [on SciFinder(R)]


Zusätzliche Organisationseinheit(en)
Lehrstuhl für Thermische Verfahrenstechnik


Einrichtungen weiterer Autorinnen und Autoren

Quantachrome Instruments
Universität Hamburg


Zitierweisen

APA:
Fried, D.I., Bednarski, D., Dreifke, M., Brieler, F.J., Thommes, M., & Froba, M. (2015). Influence of the hydrophilic-hydrophobic contrast of porous surfaces on the enzymatic performance. Journal of Materials Chemistry B, 3(11), 2341-2349-2349. https://dx.doi.org/10.1039/C4TB01700E

MLA:
Fried, Dorothee I., et al. "Influence of the hydrophilic-hydrophobic contrast of porous surfaces on the enzymatic performance." Journal of Materials Chemistry B 3.11 (2015): 2341-2349-2349.

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Zuletzt aktualisiert 2019-21-02 um 09:08