Inhibition of HIV-1 infection by human pegivirus type 1-derived peptides is affected by human pegivirus type 1 genotype and HIV-1 coreceptor tropism

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Details zur Publikation

Autor(en): Ruegamer T, Hoffmann R, Rohrhofer A, Audebert F, Salzberger B, Korn K, Schuster P, Eichler J, Schmidt B
Zeitschrift: Aids
Jahr der Veröffentlichung: 2018
Band: 32
Heftnummer: 14
Seitenbereich: 1951-1957
ISSN: 0269-9370


Abstract

Objective(s): Up to 40% of HIV-1 infected individuals are coinfected with human pegivirus type 1 (HPgV-1). The majority of studies, but not all, have reported a beneficial effect of HPgV-1 coinfection on HIV-1 disease progression. So far, the impact of different HPgV-1 genotypes on different HIV-1 subtypes remains unclear.
Methods: Peptides derived from HPgV-1 envelope protein E2, and representing different viral genotypes, were synthesized using Fmoc/t-Bu-based solid phase peptide synthesis. The inhibitory effect of these peptides on the infection of reporter cell lines was tested using an HIV-1 subtype panel representing clades A (n = 2), AG (n = 2), B (n = 6), C (n = 2), D (n = 2), F (n = 2), G (n = 1), G/H (n = 1), and group O (n = 2).
Results: HIV-1 infection was blocked more efficiently by peptides derived from HPgV-1 GT2 than GT1 (P = 0.05). The HIV-1 subtype did not affect the degree of inhibition by a peptide derived from HPgV-1 GT2. All CXCR4-/dual-tropic isolates (n = 12), but only half (four out of eight) CCR5-tropic viruses were inhibited by this peptide (P = 0.014).
Conclusion: Our data indicate that the inhibitory effect of peptides derived from HPgV-1 E2 protein is dependent on the genotype, suggesting that coinfection with HPgV-1 GT1 is less likely to confer a beneficial effect on HIV-1 disease progression than GT2. The preferential suppression of more pathogenic CXCR4-tropic HIV-1 by peptides derived from HPgV-1 GT2 may explain the favorable effect in patients harboring these HIV-1 isolates. Consequently, HPgV-1 genotype and HIV-1 coreceptor tropism are likely determinants for the beneficial effect of HPgV-1 co-infection in HIV-1-infected individuals. Copyright (C) 2018 Wolters Kluwer Health, Inc. All rights reserved.


FAU-Autoren / FAU-Herausgeber

Eichler, Jutta Prof. Dr.
Professur für Pharmazeutische Chemie
Hoffmann, Rebecca
Professur für Pharmazeutische Chemie


Autor(en) der externen Einrichtung(en)
Universität Regensburg
Universitätsklinikum Regensburg


Zitierweisen

APA:
Ruegamer, T., Hoffmann, R., Rohrhofer, A., Audebert, F., Salzberger, B., Korn, K.,... Schmidt, B. (2018). Inhibition of HIV-1 infection by human pegivirus type 1-derived peptides is affected by human pegivirus type 1 genotype and HIV-1 coreceptor tropism. Aids, 32(14), 1951-1957. https://dx.doi.org/10.1097/QAD.0000000000001926

MLA:
Ruegamer, Tamara, et al. "Inhibition of HIV-1 infection by human pegivirus type 1-derived peptides is affected by human pegivirus type 1 genotype and HIV-1 coreceptor tropism." Aids 32.14 (2018): 1951-1957.

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Zuletzt aktualisiert 2019-05-02 um 12:10