Mathematical imaging methods for mitosis analysis in live-cell phase contrast microscopy

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Grah JS, Harrington JA, Koh SB, Pike JA, Schreiner A, Burger M, Schonlieb CB, Reichelt S
Zeitschrift: Methods
Verlag: Academic Press Inc.
Jahr der Veröffentlichung: 2017
Band: null
ISSN: 1046-2023


Abstract

In this paper we propose a workflow to detect and track mitotic cells in time-lapse microscopy image sequences. In order to avoid the requirement for cell lines expressing fluorescent markers and the associated phototoxicity, phase contrast microscopy is often preferred over fluorescence microscopy in live-cell imaging. However, common specific image characteristics complicate image processing and impede use of standard methods. Nevertheless, automated analysis is desirable due to manual analysis being subjective, biased and extremely time-consuming for large data sets. Here, we present the following workflow based on mathematical imaging methods. In the first step, mitosis detection is performed by means of the circular Hough transform. The obtained circular contour subsequently serves as an initialisation for the tracking algorithm based on variational methods. It is sub-divided into two parts: in order to determine the beginning of the whole mitosis cycle, a backwards tracking procedure is performed. After that, the cell is tracked forwards in time until the end of mitosis. As a result, the average of mitosis duration and ratios of different cell fates (cell death, no division, division into two or more daughter cells) can be measured and statistics on cell morphologies can be obtained. All of the tools are featured in the user-friendly MATLAB®Graphical User Interface MitosisAnalyser.


Einrichtungen weiterer Autorinnen und Autoren

University of Cambridge
Westfälische Wilhelms-Universität (WWU) Münster


Zitierweisen

APA:
Grah, J.S., Harrington, J.A., Koh, S.B., Pike, J.A., Schreiner, A., Burger, M.,... Reichelt, S. (2017). Mathematical imaging methods for mitosis analysis in live-cell phase contrast microscopy. Methods, null. https://dx.doi.org/10.1016/j.ymeth.2017.02.001

MLA:
Grah, Joana Sarah, et al. "Mathematical imaging methods for mitosis analysis in live-cell phase contrast microscopy." Methods null (2017).

BibTeX: 

Zuletzt aktualisiert 2019-02-04 um 15:23