In situ visualization of damaged DNA in human sperm by Raman microspectroscopy

Mallidis C, Wistuba J, Bleisteiner B, Damm OS, Gross P, Wuebbeling F, Fallnich C, Burger M, Schlatt S (2011)

Publication Language: English

Publication Type: Journal article

Publication year: 2011

Journal

Human Reproduction Oxford University Press (OUP): Policy B1 - Oxford Open Option B

Book Volume: 26

Pages Range: 1641-9

Issue: 7

DOI: 10.1093/humrep/der122

Abstract

Beyond determining the percentage of damaged sperm, current methods of DNA assessment are of limited clinical utility as they render the sample unusable. We evaluated Raman microspectroscopy, a laser-based non-invasive technique that provides detailed chemical 'fingerprints' of cells and which potentially could be used for nuclear DNA-based sperm selection.Eight healthy donors provided ejaculates. After system optimization, a minimum of 200 air-dried sperm/sample/donor, prior to/and after UVB irradiation, were assessed by two observers. Spectra were analysed by Principal Component, Spectral Angle and Wavelet Analyses.Spectra provided a chemical map delineating each sperm head region. Principal Component Analysis showed clear separation between spectra from UV-irradiated and untreated samples whilst averaged data identified two regions of interest (1040 and 1400 cm(-1)). Local spectral analysis around the DNA PO(4) backbone peak (1042 cm(-1)), showed that changes in this region were indicative of DNA damage. Wavelet decomposition confirmed both the 1042 cm(-1) shift and a second UVB susceptible region (1400-1600 cm(-1)) corresponding to protein-DNA interactions. No difference was found between observer measurements.Raman microspectroscopy can provide accurate and reproducible assessment of sperm DNA structure and the sites and location of damage.

Authors with CRIS profile

Martin Burger

Involved external institutions

HORIBA Jobin Yvon GmbH DE Germany (DE) Westfälische Wilhelms-Universität (WWU) Münster DE Germany (DE)

How to cite

APA:

Mallidis, C., Wistuba, J., Bleisteiner, B., Damm, O.S., Gross, P., Wuebbeling, F.,... Schlatt, S. (2011). In situ visualization of damaged DNA in human sperm by Raman microspectroscopy. Human Reproduction, 26, 1641-9. https://doi.org/10.1093/humrep/der122

MLA:

Mallidis, Con, et al. "In situ visualization of damaged DNA in human sperm by Raman microspectroscopy." Human Reproduction 26 (2011): 1641-9.

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