In situ visualization of damaged DNA in human sperm by Raman microspectroscopy

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Details zur Publikation

Autorinnen und Autoren: Mallidis C, Wistuba J, Bleisteiner B, Damm OS, Gross P, Wuebbeling F, Fallnich C, Burger M, Schlatt S
Zeitschrift: Human Reproduction
Jahr der Veröffentlichung: 2011
Band: 26
Seitenbereich: 1641-9
ISSN: 0268-1161
Sprache: Englisch


Abstract

Beyond determining the percentage of damaged sperm, current methods of DNA assessment are of limited clinical utility as they render the sample unusable. We evaluated Raman microspectroscopy, a laser-based non-invasive technique that provides detailed chemical 'fingerprints' of cells and which potentially could be used for nuclear DNA-based sperm selection.Eight healthy donors provided ejaculates. After system optimization, a minimum of 200 air-dried sperm/sample/donor, prior to/and after UVB irradiation, were assessed by two observers. Spectra were analysed by Principal Component, Spectral Angle and Wavelet Analyses.Spectra provided a chemical map delineating each sperm head region. Principal Component Analysis showed clear separation between spectra from UV-irradiated and untreated samples whilst averaged data identified two regions of interest (1040 and 1400 cm(-1)). Local spectral analysis around the DNA PO(4) backbone peak (1042 cm(-1)), showed that changes in this region were indicative of DNA damage. Wavelet decomposition confirmed both the 1042 cm(-1) shift and a second UVB susceptible region (1400-1600 cm(-1)) corresponding to protein-DNA interactions. No difference was found between observer measurements.Raman microspectroscopy can provide accurate and reproducible assessment of sperm DNA structure and the sites and location of damage.


Einrichtungen weiterer Autorinnen und Autoren

HORIBA Jobin Yvon GmbH
Westfälische Wilhelms-Universität (WWU) Münster


Zitierweisen

APA:
Mallidis, C., Wistuba, J., Bleisteiner, B., Damm, O.S., Gross, P., Wuebbeling, F.,... Schlatt, S. (2011). In situ visualization of damaged DNA in human sperm by Raman microspectroscopy. Human Reproduction, 26, 1641-9. https://dx.doi.org/10.1093/humrep/der122

MLA:
Mallidis, Con, et al. "In situ visualization of damaged DNA in human sperm by Raman microspectroscopy." Human Reproduction 26 (2011): 1641-9.

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Zuletzt aktualisiert 2018-03-12 um 16:08