Hyaluronic acid based extracellular matrix regulates surface expression of GluN2B containing NMDA receptors

Schweitzer B, Singh J, Fejtova A, Groc L, Heine M, Frischknecht R (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 7

Journal Issue: 1

DOI: 10.1038/s41598-017-07003-3

Abstract

Cortical areas of the juvenile rodent brain display a high degree of structural and functional plasticity, which disappears later in development. Coincident with the decline of plasticity 1) the hyaluronic acid-based extracellular matrix (ECM) of the brain, which stabilizes synapses and neuronal circuit is formed and 2) N-methyl-D-aspartate subtype of ionotropic glutamate receptors (NMDARs) implied in synaptic plasticity switch from mainly GluN2B to GluN2A subunit-containing receptors. Here we tested the hypothesis that ECM influences the NMDAR subunit composition in dissociated neuronal cultures. Experimental removal of ECM using hyaluronidase induced an increase in surface expression of GluN2B. This was due to decreased endocytosis of surface GluNB-containing receptors. We further found a reduction in phosphorylation at Tyr1472, which negatively regulates their binding to the endocytotic AP2 complex. We propose that maturation of ECM could induce switch in NMDAR composition necessary for normal adult synaptic plasticity and that increased expression of GluN2B contributes to rejuvenation of plasticity after ECM removal in vivo.

Authors with CRIS profile

Renato Frischknecht Lehrstuhl für Tierphysiologie

Additional Organisation(s)

Department of Psychiatry and Psychotherapy

Involved external institutions

Max-Planck-Institut für Neurobiologie / Max Planck Institute of Neurobiology DE Germany (DE) Interdisciplinary Institute for Neuroscience IINS Bordeaux neurocampus FR France (FR)

How to cite

APA:

Schweitzer, B., Singh, J., Fejtova, A., Groc, L., Heine, M., & Frischknecht, R. (2017). Hyaluronic acid based extracellular matrix regulates surface expression of GluN2B containing NMDA receptors. Scientific Reports, 7(1). https://dx.doi.org/10.1038/s41598-017-07003-3

MLA:

Schweitzer, Barbara, et al. "Hyaluronic acid based extracellular matrix regulates surface expression of GluN2B containing NMDA receptors." Scientific Reports 7.1 (2017).

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