Inflammatory pain control by blocking oxidized phospholipid-mediated TRP channel activation

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Oehler B, Kistner K, Martin C, Schiller J, Mayer R, Mohammadi M, Sauer RS, Filipovic M, Nieto FR, Kloka J, Pfluecke D, Hill K, Schaefer M, Malcangio M, Reeh P, Brack A, Blum R, Rittner HL
Zeitschrift: Scientific Reports
Jahr der Veröffentlichung: 2017
Band: 7
Heftnummer: 1
ISSN: 2045-2322


Abstract

Phospholipids occurring in cell membranes and lipoproteins are converted into oxidized phospholipids (OxPL) by oxidative stress promoting atherosclerotic plaque formation. Here, OxPL were characterized as novel targets in acute and chronic inflammatory pain. Oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (OxPAPC) and its derivatives were identified in inflamed tissue by mass spectrometry and binding assays. They elicited calcium influx, hyperalgesia and induced pro-nociceptive peptide release. Genetic, pharmacological and mass spectrometric evidence in vivo as well as in vitro confirmed the role of transient receptor potential channels (TRPA1 and TRPV1) as OxPAPC targets. Treatment with the monoclonal antibody E06 or with apolipoprotein A-I mimetic peptide D-4F, capturing OxPAPC in atherosclerosis, prevented inflammatory hyperalgesia, and in vitro TRPA1 activation. Administration of D-4F or E06 to rats profoundly ameliorated mechanical hyperalgesia and inflammation in collagen-induced arthritis. These data reveal a clinically relevant role for OxPAPC in inflammation offering therapy for acute and chronic inflammatory pain treatment by scavenging OxPAPC.


FAU-Autoren / FAU-Herausgeber

Filipovic, Milos
Lehrstuhl für Bioanorganische Chemie
Kistner, Katrin Dr.
Lehrstuhl für Physiologie
Reeh, Peter Prof. Dr.
Medizinische Fakultät


Autor(en) der externen Einrichtung(en)
King’s College London
Universität Leipzig
Universitätsklinikum Würzburg


Zitierweisen

APA:
Oehler, B., Kistner, K., Martin, C., Schiller, J., Mayer, R., Mohammadi, M.,... Rittner, H.L. (2017). Inflammatory pain control by blocking oxidized phospholipid-mediated TRP channel activation. Scientific Reports, 7(1). https://dx.doi.org/10.1038/s41598-017-05348-3

MLA:
Oehler, Beatrice, et al. "Inflammatory pain control by blocking oxidized phospholipid-mediated TRP channel activation." Scientific Reports 7.1 (2017).

BibTeX: 

Zuletzt aktualisiert 2018-10-10 um 13:23