Phosphoglucose isomerase deficiency in Escherichia coli K-12 is associated with increased spontaneous mutation rate

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autor(en): Boteva E, Handzhiyski Y, Kotseva M, Datsenko KA, Wanner BL, Pischetsrieder M, Mironova R
Zeitschrift: Advances in Microbiology
Jahr der Veröffentlichung: 2018
Band: 8
Seitenbereich: 390-405
ISSN: 2165-3402
eISSN: 2165-3410


Abstract

Phosphoglucose isomerase (PGI) is a key enzyme in early glycolysis, which
catalyzes the reversible isomerization of glucose 6-phosphate (G6Ph) to fructose
6-phosphate. We have constructed an Escherichia coli K12 strain with a
deleted pgi gene (Δpgi ) and shown that this strain in comparison with the parental
strain 1) accumulates higher amount of G6Ph, 2) grows slowly, and 3)
exhibits higher spontaneous mutation frequency to rifampicin resistance
(Rifr), when grown on high glucose minimal medium. Intriguingly, the spontaneous
mutation rate to Rifr was inversely related to the degree of E. coli
chromosomal DNA modification with sugar derivatives. We measured higher
concentrations of Amadori products, fluorophores (360 nm excitation/440
nm emission) and carboxymethyl residues in the chromosomal DNA of the E.
coli parental strain than in DNA of the isogenic Δpgi strain. To explain this
apparent paradox we hypothesized that PGI might be implicated in repair of
G6Ph-derived lesions in DNA. In favor of our hypothesis, we further demonstrate
that protein extract from the E. coli PGI proficient strain but not from
the PGI deficient strain catalyzes the release of G6Ph from G6Ph-modified
single stranded DNA oligonucleotide and from its hybrid duplex with a complementary
peptide nucleic acid.


FAU-Autoren / FAU-Herausgeber

Pischetsrieder, Monika Prof. Dr.
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)


Autor(en) der externen Einrichtung(en)
Bulgarian Academy of Sciences (BAS) / Българска академия на науките
Harvard University
Purdue-University


Zitierweisen

APA:
Boteva, E., Handzhiyski, Y., Kotseva, M., Datsenko, K.A., Wanner, B.L., Pischetsrieder, M., & Mironova, R. (2018). Phosphoglucose isomerase deficiency in Escherichia coli K-12 is associated with increased spontaneous mutation rate. Advances in Microbiology, 8, 390-405. https://dx.doi.org/10.4236/aim.2018.85026

MLA:
Boteva, Elitsa, et al. "Phosphoglucose isomerase deficiency in Escherichia coli K-12 is associated with increased spontaneous mutation rate." Advances in Microbiology 8 (2018): 390-405.

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Zuletzt aktualisiert 2019-05-01 um 07:10