Phosphoglucose isomerase deficiency in Escherichia coli K-12 is associated with increased spontaneous mutation rate

Boteva E, Handzhiyski Y, Kotseva M, Datsenko KA, Wanner BL, Pischetsrieder M, Mironova R (2018)

Publication Type: Journal article, Original article

Publication year: 2018

Journal

Advances in Microbiology Scientific Research Publishing

Book Volume: 8

Pages Range: 390-405

URI: https://file.scirp.org/pdf/AiM_2018052914543097.pdf

DOI: 10.4236/aim.2018.85026

Abstract

Phosphoglucose isomerase (PGI) is a key enzyme in early glycolysis, which
catalyzes the reversible isomerization of glucose 6-phosphate (G6Ph) to fructose
6-phosphate. We have constructed an Escherichia coli K12 strain with a
deleted pgi gene (Δpgi ) and shown that this strain in comparison with the parental
strain 1) accumulates higher amount of G6Ph, 2) grows slowly, and 3)
exhibits higher spontaneous mutation frequency to rifampicin resistance
(Rifr), when grown on high glucose minimal medium. Intriguingly, the spontaneous
mutation rate to Rifr was inversely related to the degree of E. coli
chromosomal DNA modification with sugar derivatives. We measured higher
concentrations of Amadori products, fluorophores (360 nm excitation/440
nm emission) and carboxymethyl residues in the chromosomal DNA of the E.
coli parental strain than in DNA of the isogenic Δpgi strain. To explain this
apparent paradox we hypothesized that PGI might be implicated in repair of
G6Ph-derived lesions in DNA. In favor of our hypothesis, we further demonstrate
that protein extract from the E. coli PGI proficient strain but not from
the PGI deficient strain catalyzes the release of G6Ph from G6Ph-modified
single stranded DNA oligonucleotide and from its hybrid duplex with a complementary
peptide nucleic acid.

Authors with CRIS profile

Monika Pischetsrieder Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)

Involved external institutions

Harvard University US United States (USA) (US) Bulgarian Academy of Sciences (BAS) / Българска академия на науките BG Bulgaria (BG) Purdue-University US United States (USA) (US)

How to cite

APA:

Boteva, E., Handzhiyski, Y., Kotseva, M., Datsenko, K.A., Wanner, B.L., Pischetsrieder, M., & Mironova, R. (2018). Phosphoglucose isomerase deficiency in Escherichia coli K-12 is associated with increased spontaneous mutation rate. Advances in Microbiology, 8, 390-405. https://dx.doi.org/10.4236/aim.2018.85026

MLA:

Boteva, Elitsa, et al. "Phosphoglucose isomerase deficiency in Escherichia coli K-12 is associated with increased spontaneous mutation rate." Advances in Microbiology 8 (2018): 390-405.

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