Rodent’s Stride Length Depends on Body Size: Implications for CatWalk Assay

Beitrag bei einer Tagung

Details zur Publikation

Autor(en): Timotius I, Moceri S, Plank AC, Habermeyer J, Canneva F, Casadei N, Riess O, Winkler J, Klucken J, Eskofier B, von Hörsten S
Herausgeber: Robyn Grant, Tom Allen, Andrew Spink, Matthew Sullivan
Verlagsort: UK
Jahr der Veröffentlichung: 2018
Tagungsband: Measuring Behavior 2018
ISBN: 978-1-910029-39-8
Sprache: Englisch


The analysis of gait
is an essential part of rodent phenotyping. Regarding the complex nature of
walking, preclinical gait analysis is normally based on several gait parameters
(which have been progressively developed [1,2] in recent years) including the animal’s stride
length, body speed, support, etc. The stride length is altered in several
diseases [3–5] and this parameter can be easily translated
from rodent models to human patients. However, we hypothesized that the stride length
is affected by the body size [6,7], which becomes a challenge in analyzing gait.

Using the gait
parameters assessed by CatWalk system [8–10], we examined the correlation between body-length/body-weight
with stride length using CatWalk-video-derived body silhouette length. To examine
the effect of growth and aging in rodents, we studied wild-type Sprague-Dawley
male rats and C57BL/6N mice. Moreover, we examined the body size difference
between genotypes in BACSCNA transgenic rats [11,12] and BACHD transgenic mice [13]. The CatWalk data were collected as the rodents
walked freely on top of a glass-floored corridor. The rat gait was monitored at
4 different age points (10, 26, 55 and 62 weeks old), whereas the mice were
monitored at 3 different age points (20, 32, and 47 weeks old). The rodent
numbers included in the experiments were: (a) wild-type male rats (n=16-27/time point), (b) BACSCNA rats (n=19-32/time point), (c) wild-type mice
(n=12-13/time point), and (d) BACHD
mice (n=9-11/time point). The rodents
were maintained under specific-pathogen-free condition. All research and animal
care procedures were performed in compliance with international animal welfare
standards and approved by the district governments of Lower Franconia, Würzburg,
Bavaria, Germany (RegUFr#55.2-2532-2-218).

The correlations
between front stride length (averaged from the left and right side) and the
body-silhouette-length/body-weight are shown in Figure 1. Significant
correlations are shown with both body silhouette lengths and body weight. The
correlation between hind stride length and body size showed the similar
relationship as the front stride length (data not shown). Besides body size
differences due to growth, pathological body size differences were also
observed as shown in Figure 2.

Thus, we demonstrated
that stride length is highly correlated with body silhouette length (based on the
CatWalk video) and body weight. In the near future, we need to subject gait
parameters to a scaling process using body weight or silhouette length, raising
the possibility of normalization for differences in size as a potential
confounding of gait measures.

FAU-Autoren / FAU-Herausgeber

Eskofier, Björn Prof. Dr.
Lehrstuhl für Informatik 14 (Maschinelles Lernen und Datenanalytik)
Habermeyer, Johanna
Professur für Experimentelle Biomedizin
Klucken, Jochen Prof. Dr.
Molekular-Neurologische Abteilung in der Neurologischen Klinik
Moceri, Sandra
Professur für Experimentelle Biomedizin
Plank, Anne-Christine
Professur für Experimentelle Biomedizin
Timotius, Ivanna
Lehrstuhl für Informatik 14 (Maschinelles Lernen und Datenanalytik)
von Hörsten, Stephan Prof. Dr.
Professur für Experimentelle Biomedizin
Winkler, Jürgen Prof. Dr.
Professur für Molekulare Neurologie

Autor(en) der externen Einrichtung(en)
Eberhard Karls Universität Tübingen


Timotius, I., Moceri, S., Plank, A.-C., Habermeyer, J., Canneva, F., Casadei, N.,... von Hörsten, S. (2018). Rodent’s Stride Length Depends on Body Size: Implications for CatWalk Assay. In Robyn Grant, Tom Allen, Andrew Spink, Matthew Sullivan (Eds.), Measuring Behavior 2018. Manchester, GB: UK.

Timotius, Ivanna, et al. "Rodent’s Stride Length Depends on Body Size: Implications for CatWalk Assay." Proceedings of the 11th International Conference on Methods and Techniques in Behavioral Research (Measuring Behavior 2018), Manchester Ed. Robyn Grant, Tom Allen, Andrew Spink, Matthew Sullivan, UK, 2018.


Zuletzt aktualisiert 2018-11-08 um 02:55