Rheological examination and 3D bioprinting of pre-gelled alginate hydrogels

Hazur J, Kaschta J, Bednarzig V, Boccaccini AR, Schubert DW, Detsch R (2018)

Publication Type: Conference contribution, Abstract of a poster

Publication year: 2018

Event location: Kopenhagen DK

Abstract

lginate hydrogels are from special interest in wound healing, drug delivery and tissue engineering applications, due to their biocompatibility and similarity to the extracellular matrices in tissues [1]. In this project, bioprinting of pre-crosslinked medical grade alginate gels via an internal gelation method using D-(+)-Gluconic acid-lactone in combination with CaCO3 was implemented and the rheological characteristics of the gels were examined.

By parameter optimization, it was possible to print 3D porous structures with a height of 5 mm and a pore size of 1.1 mm with those gels. Surprisingly, the stirring process during cross-linking turned out to play a major role for the absolute value of complex viscosity (|η*|), as well as for the homogeneity of the gels. A significant increase of |η*| as well as of the storage modulus was obtained, when the gels were not stirred, leading to gels without viscous flow and therefore to less defined printed structures.

Apart from that, the temperature during pre-crosslinking plays an important role on the homogeneity. At lower temperatures than room temperature, the gels showed a more uniform gelation process, compared to those at 20 °C.
Furthermore, the concentration and temperature dependence of the alginate solutions were rheologically characterized. The shear dependent viscosities were superposable for both temperature and concentration and appropriate fitting functions in the investigated regions were derived.

Authors with CRIS profile

Jonas Hazur Sonderforschungsbereich/Transregio 225/2: Von den Grundlagen der Biofabrikation zu funktionalen Gewebemodellen Joachim Kaschta Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Vera Bednarzig Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Dirk Schubert Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Rainer Detsch Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

How to cite

APA:

Hazur, J., Kaschta, J., Bednarzig, V., Boccaccini, A.R., Schubert, D.W., & Detsch, R. (2018, May). Rheological examination and 3D bioprinting of pre-gelled alginate hydrogels. Poster presentation at Nordic Polymer Days 2018, Kopenhagen, DK.

MLA:

Hazur, Jonas, et al. "Rheological examination and 3D bioprinting of pre-gelled alginate hydrogels." Presented at Nordic Polymer Days 2018, Kopenhagen 2018.

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