Toxicity of Mitoxantrone-loaded Superparamagnetic Iron Oxide Nanoparticles in a HT-29 Tumour Spheroid Model

Hornung A, Poettler M, Friedrich RP, Weigel B, Dürr S, Zaloga J, Cicha I, Alexiou C, Janko C (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Anticancer Research International Institute of Anticancer Research (IIAR)

Book Volume: 36

Pages Range: 3093-101

Journal Issue: 6

Abstract

Cancer research is commonly carried out in two-dimensional (2D) cell cultures, which poorly reflect in vivo settings where the growing tumours are exposed to mechanical forces and biochemical gradients. In this study we established a HF-29 colon carcinoma tumor spheroid model to investigate the effect of free mitoxantrone (MTO) and its nanoparticle-bound form (SPION(MTO)) under 3D cell culture conditions.Tumour spheroids were generated by seeding HT-29 colon carcinoma cells on agarose-coated cell culture wells. Growth of the spheroids was monitored daily by transmission microscopy upon treatment with free MTO, SPION(MTO) or unloaded SPION.Unloaded SPION did not affect the spheroid size compared to untreated controls, while both free MTO and SPION(MTO) inhibited growth of the spheroids in a dose- and time-dependent manner. In comparison to free MTO, the effect of SPION(MTO) on spheroid growth was slightly delayed. Further analyses are necessary to investigate if MTO infiltrates spheroids in its nanoparticle-bound form or whether it is released from SPION before infiltration.

Authors with CRIS profile

Stephan Dürr Lehrstuhl für Hals-, Nasen- und Ohrenheilkunde Jan Zaloga Department of Otorhinolaryngology – Head and Neck Surgery Iwona Cicha Lehrstuhl für Innere Medizin II Christoph Alexiou Professur für Nanomedizin Christina Janko Department of Otorhinolaryngology – Head and Neck Surgery

Additional Organisation(s)

Exzellenz-Cluster Engineering of Advanced Materials

How to cite

APA:

Hornung, A., Poettler, M., Friedrich, R.P., Weigel, B., Dürr, S., Zaloga, J.,... Janko, C. (2016). Toxicity of Mitoxantrone-loaded Superparamagnetic Iron Oxide Nanoparticles in a HT-29 Tumour Spheroid Model. Anticancer Research, 36(6), 3093-101.

MLA:

Hornung, Annkathrin, et al. "Toxicity of Mitoxantrone-loaded Superparamagnetic Iron Oxide Nanoparticles in a HT-29 Tumour Spheroid Model." Anticancer Research 36.6 (2016): 3093-101.

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