Non-invasive F-actin visualization in living plant cells using a GFP mouse talin fusion protein
Kost B, Spielhofer P, Mathur J, Dong CH, Chua NH (2000)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2000
Publisher: Springer Netherlands
Edited Volumes: Actin: a dynamic framework for multiple plant cell functions
Series: Developments in Plant and Soil Sciences
City/Town: Dordrecht
Book Volume: 89
Pages Range: 637-659
ISBN: 978-90-481-5504-0
DOI: 10.1007/978-94-015-9460-8_36
Abstract
The actin cytoskeleton has essential functions in the survival, the division, the growth and the morphogenesis of plant cells, and in the development of plant organs. A key approach to investigating these functions is the observation of the organization and the dynamic behavior of filamentous actin structures in plants cells. Visualizing plant actin filaments has been notoriously difficult, because they are highly sensitive to standard fixation and cell permeabilization procedures. This chapter demonstrates that the expression in plant cells of an optimized mutant version of the green fluorescent protein (GFP) from Aequorea victoria fused to the well characterized F-actin binding domain of mouse talin labels the actin cytoskeleton and allows non-invasive observation of its dynamic organization by fluorescence microscopy.
Authors with CRIS profile
Involved external institutions
National University of Singapore (NUS)
Singapore (SG)
Rockefeller University
United States (USA) (US)
Singapore (SG)
Rockefeller University
United States (USA) (US)
How to cite
APA:
Kost, B., Spielhofer, P., Mathur, J., Dong, C.-H., & Chua, N.-H. (2000). Non-invasive F-actin visualization in living plant cells using a GFP mouse talin fusion protein. In C. J. Staiger, F. Baluška,D. Volkmann, P. W. Barlow (Eds.), Actin: a dynamic framework for multiple plant cell functions. (pp. 637-659). Dordrecht: Springer Netherlands.
MLA:
Kost, Benedikt, et al. "Non-invasive F-actin visualization in living plant cells using a GFP mouse talin fusion protein." Actin: a dynamic framework for multiple plant cell functions. Ed. C. J. Staiger, F. Baluška,D. Volkmann, P. W. Barlow, Dordrecht: Springer Netherlands, 2000. 637-659.
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