Metabolite profiling of barley flag leaves under drought and combined heat and drought stress reveals metabolic QTLs for metabolites associated with antioxidant defense

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Templer SE, Ammon A, Pscheidt D, Ciobotea O, Schuy C, Mccollum C, Sonnewald U, Hanemann A, Foerster J, Ordon F, Von Korff M, Voll L
Zeitschrift: Journal of Experimental Botany
Verlag: OXFORD UNIV PRESS
Jahr der Veröffentlichung: 2017
Band: 68
Heftnummer: 7
Seitenbereich: 1697-1713
ISSN: 0022-0957


Abstract


Barley (Hordeum vulgare L.) is among the most stress-tolerant crops; however, not much is known about the genetic and environmental control of metabolic adaptation of barley to abiotic stresses. We have subjected a genetically diverse set of 81 barley accessions, consisting of Mediterranean landrace genotypes and German elite breeding lines, to drought and combined heat and drought stress at anthesis. Our aim was to (i) investigate potential differences in morphological, physiological, and metabolic adaptation to the two stress scenarios between the Mediterranean and German barley genotypes and (ii) identify metabolic quantitative trait loci (mQTLs). To this end, we have genotyped the investigated barley lines with an Illumina iSelect 9K array and analyzed a set of 57 metabolites from the primary C and N as well as antioxidant metabolism in flag leaves under control and stress conditions. We found that drought-adapted genotypes attenuate leaf carbon metabolism much more strongly than elite lines during drought stress adaptation. Furthermore, we identified mQTLs for flag leaf gamma-tocopherol, glutathione, and succinate content by association genetics that co-localize with genes encoding enzymes of the pathways producing these antioxidant metabolites. Our results provide the molecular basis for breeding barley cultivars with improved abiotic stress tolerance.



FAU-Autoren / FAU-Herausgeber

Ammon, Alexandra
Lehrstuhl für Biochemie
Ciobotea, Otilia
Lehrstuhl für Biochemie
Pscheidt, David
Lehrstuhl für Biochemie
Schuy, Christian
Lehrstuhl für Biochemie
Sonnewald, Uwe Prof. Dr.
Lehrstuhl für Biochemie
Voll, Lars PD Dr.
Lehrstuhl für Biochemie


Autor(en) der externen Einrichtung(en)
Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen (JKI)
Max-Planck-Institut für Pflanzenzüchtungsforschung / Max Planck Institute for Plant Breeding Research
Saaten-Union Biotec GmbH
Saatzucht Josef Breun GmbH & Co. KG


Zitierweisen

APA:
Templer, S.E., Ammon, A., Pscheidt, D., Ciobotea, O., Schuy, C., Mccollum, C.,... Voll, L. (2017). Metabolite profiling of barley flag leaves under drought and combined heat and drought stress reveals metabolic QTLs for metabolites associated with antioxidant defense. Journal of Experimental Botany, 68(7), 1697-1713. https://dx.doi.org/10.1093/jxb/erx038

MLA:
Templer, Sven Eduard, et al. "Metabolite profiling of barley flag leaves under drought and combined heat and drought stress reveals metabolic QTLs for metabolites associated with antioxidant defense." Journal of Experimental Botany 68.7 (2017): 1697-1713.

BibTeX: 

Zuletzt aktualisiert 2018-10-08 um 21:30