Metabolite profiling of barley flag leaves under drought and combined heat and drought stress reveals metabolic QTLs for metabolites associated with antioxidant defense

Templer SE, Ammon A, Pscheidt D, Ciobotea O, Schuy C, Mccollum C, Sonnewald U, Hanemann A, Foerster J, Ordon F, Von Korff M, Voll L (2017)

Publication Status: Published

Publication Type: Journal article

Publication year: 2017

Journal

Publisher: OXFORD UNIV PRESS

Book Volume: 68

Pages Range: 1697-1713

Journal Issue: 7

DOI: 10.1093/jxb/erx038

Abstract

Barley (Hordeum vulgare L.) is among the most stress-tolerant crops; however, not much is known about the genetic and environmental control of metabolic adaptation of barley to abiotic stresses. We have subjected a genetically diverse set of 81 barley accessions, consisting of Mediterranean landrace genotypes and German elite breeding lines, to drought and combined heat and drought stress at anthesis. Our aim was to (i) investigate potential differences in morphological, physiological, and metabolic adaptation to the two stress scenarios between the Mediterranean and German barley genotypes and (ii) identify metabolic quantitative trait loci (mQTLs). To this end, we have genotyped the investigated barley lines with an Illumina iSelect 9K array and analyzed a set of 57 metabolites from the primary C and N as well as antioxidant metabolism in flag leaves under control and stress conditions. We found that drought-adapted genotypes attenuate leaf carbon metabolism much more strongly than elite lines during drought stress adaptation. Furthermore, we identified mQTLs for flag leaf gamma-tocopherol, glutathione, and succinate content by association genetics that co-localize with genes encoding enzymes of the pathways producing these antioxidant metabolites. Our results provide the molecular basis for breeding barley cultivars with improved abiotic stress tolerance.

Authors with CRIS profile

Involved external institutions

Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen (JKI) Germany (DE) Saatzucht Josef Breun GmbH & Co. KG Germany (DE) Saaten-Union Biotec GmbH Germany (DE) Max-Planck-Institut für Pflanzenzüchtungsforschung / Max Planck Institute for Plant Breeding Research Germany (DE)

How to cite

APA:

Templer, S.E., Ammon, A., Pscheidt, D., Ciobotea, O., Schuy, C., Mccollum, C.,... Voll, L. (2017). Metabolite profiling of barley flag leaves under drought and combined heat and drought stress reveals metabolic QTLs for metabolites associated with antioxidant defense. Journal of Experimental Botany, 68(7), 1697-1713. https://dx.doi.org/10.1093/jxb/erx038

MLA:

Templer, Sven Eduard, et al. "Metabolite profiling of barley flag leaves under drought and combined heat and drought stress reveals metabolic QTLs for metabolites associated with antioxidant defense." Journal of Experimental Botany 68.7 (2017): 1697-1713.

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