Evidence that Doublecortin is dispensable for the development of adult born neurons in mice
Merz K, Lie DC (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 8
Pages Range: e62693
Journal Issue: 5
DOI: 10.1371/journal.pone.0062693
Abstract
In mammals, adult neural stem cells give rise to new hippocampal dentate granule neurons and interneurons of the olfactory bulb throughout life. The microtubule associated protein Doublecortin (DCX) is expressed by migrating neuroblasts and immature neurons, and is widely used as a stage-specific marker of adult neurogenesis and as a marker to identify neurogenic activity in the adult brain per se. Mutations in the DCX gene have been causally linked to human lissencephalic syndromes. Moreover, embryonic loss of DCX function interferes with neuronal migration and dendritic patterning in a species- and region-specific manner. A putative function of DCX in adult neurogenesis has not been directly explored. Here we show that overexpression of DCX in newly generated dentate granule neurons of the adult mouse brain has no effect on morphological maturation or migration. We also show that micro (mi) RNA-mediated retroviral knockdown of DCX does not alter morphological maturation of adult born dentate granule cells or migration of new neurons in either adult neurogenic niche. Thus, the present data indicate that DCX is dispensable for the development of new neurons in adult mice.
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Germany (DE)How to cite
APA:
Merz, K., & Lie, D.C. (2013). Evidence that Doublecortin is dispensable for the development of adult born neurons in mice. PLoS ONE, 8(5), e62693. https://doi.org/10.1371/journal.pone.0062693
MLA:
Merz, Katharina, and Dieter Chichung Lie. "Evidence that Doublecortin is dispensable for the development of adult born neurons in mice." PLoS ONE 8.5 (2013): e62693.
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