A homologue of cysteine-rich secretory proteins induces premature degradation of vitelline envelopes and hatching of Xenopus laevis embryos.

Schambony A, Hefele J, Gentzel M, Wilm M, Wedlich D (2003)

Publication Type: Journal article, Original article

Subtype: other

Publication year: 2003

Journal

Mechanisms of Development Elsevier

Publisher: Elsevier Ireland Ltd

Book Volume: 120

Pages Range: 937-948

Journal Issue: 8

URI: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=12963113&retmode=ref&cmd=prlinks

DOI: 10.1016/S0925-4773(03)00162-X

Abstract

We cloned Xenopus laevis CRISP, XCRISP, a homologue of the mammalian family of cysteine-rich secretory proteins (CRISPs), which has been previously identified as a Wnt3a/noggin responsive gene in an expression screen [Mech. Dev. 87 (1999) 21]. We detected XCRISP expression exclusively in the hatching gland. XCRISP enters the secretory pathway and accumulates on the surface of presumptive hatching gland cells. Overexpression studies of XCRISP and XCRISP-mutants show that XCRISP induces premature hatching of embryos preceded by degradation of the vitelline envelope. A deletion mutant that lacks a 35 amino acid domain even accelerates hatching, while further deletion of the carboxy-terminus reverses these effects. From our studies, we conclude that XCRISP is sufficient to induce degradation of vitelline envelopes and that this activity maps to the most C-terminal amino acids, while the adjacent domain regulates XCRISP activity.

Authors with CRIS profile

Alexandra Schambony Lehrstuhl für Entwicklungsbiologie

Involved external institutions

European Molecular Biology Laboratory (EMBL) DE Germany (DE) Karlsruhe Institute of Technology (KIT) DE Germany (DE)

How to cite

APA:

Schambony, A., Hefele, J., Gentzel, M., Wilm, M., & Wedlich, D. (2003). A homologue of cysteine-rich secretory proteins induces premature degradation of vitelline envelopes and hatching of Xenopus laevis embryos. Mechanisms of Development, 120(8), 937-948. https://doi.org/10.1016/S0925-4773(03)00162-X

MLA:

Schambony, Alexandra, et al. "A homologue of cysteine-rich secretory proteins induces premature degradation of vitelline envelopes and hatching of Xenopus laevis embryos." Mechanisms of Development 120.8 (2003): 937-948.

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