The influence of 2 kbar pressure on the global and internal dynamics of human hemoglobin observed by quasielastic neutron scattering

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Details zur Publikation

Autor(en): Appavou MS, Busch S, Doster W, Gaspar AM, Unruh T
Zeitschrift: European Biophysics Journal With Biophysics Letters
Verlag: SPRINGER
Jahr der Veröffentlichung: 2011
Band: 40
Heftnummer: 6
Seitenbereich: 705-714
ISSN: 0175-7571


Abstract


Pressure is a ubiquitous physical parameter in life and is commonly used in the life sciences to study new protein folding pathways or association-dissociation phenomena. In this paper, an investigation of the influence of pressure on hemoglobin, a multimeric protein, at the picosecond time scale is presented using time-of-flight neutron scattering. The aim is to observe the influence of pressure on the translational diffusion and internal motions of hemoglobin in a concentrated solution and a possible dissociation of the subunits as suggested by Pin et al. (Biochemistry 29:9194, 1990) using fluorescence spectroscopy. A new flat 2 kbar pressure cell made of an aluminum alloy has been used, which allowed the effect of pressure to be studied with minimum background contribution. Within this range of pressure, the effect of this physical parameter on global diffusion can be explained in terms of the change in the water buffer viscosity and an oligomerization of hemoglobin subunits, whereas the internal motions were less affected.



FAU-Autoren / FAU-Herausgeber

Unruh, Tobias Prof. Dr.
Professur für Nanomaterialcharakterisierung (Streumethoden)


Autor(en) der externen Einrichtung(en)
Forschungszentrum Jülich GmbH (FZJ)
Technische Universität München (TUM)


Zitierweisen

APA:
Appavou, M.-S., Busch, S., Doster, W., Gaspar, A.M., & Unruh, T. (2011). The influence of 2 kbar pressure on the global and internal dynamics of human hemoglobin observed by quasielastic neutron scattering. European Biophysics Journal With Biophysics Letters, 40(6), 705-714. https://dx.doi.org/10.1007/s00249-011-0678-3

MLA:
Appavou, Marie-Sousai, et al. "The influence of 2 kbar pressure on the global and internal dynamics of human hemoglobin observed by quasielastic neutron scattering." European Biophysics Journal With Biophysics Letters 40.6 (2011): 705-714.

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Zuletzt aktualisiert 2018-25-08 um 07:13