NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases

Journal article


Publication Details

Author(s): Potocky M, Pejchar P, Gutkowska M, Jose Jimenez-Quesada M, Potocka A, De Dios Alche J, Kost B, Zarsky V
Journal: Journal of Plant Physiology
Publisher: Elsevier
Publication year: 2012
Volume: 169
Journal issue: 16
Pages range: 1654-1663
ISSN: 0176-1617


Abstract


Reactive oxygen species (ROS) generated by NADPH oxidase (NOX) are crucial for tip growth of pollen tubes. However, the regulation of NOX activity in pollen tubes remains unknown. Using purified plasma membrane fractions from tobacco and olive pollen and tobacco BY-2 cells, we demonstrate that pollen NOX is activated by calcium ions and low abundant signaling phospholipids, such as phosphatidic acid and phosphatidylinositol 4,5-bisphosphate in vitro and in vivo. Our data also suggest possible synergism between Ca2+ and phospholipid-mediated NOX activation in pollen. Rac/Rop small GTPases are also necessary for normal pollen tube growth and have been proposed to regulate ROS production in root hairs. We show here elevated ROS formation in pollen tubes overexpressing wild-type NtRac5 and constitutively active NtRac5, while overexpression of dominant-negative NtRac5 led to a decrease of ROS in pollen tubes. We also show that PA formed by distinct phospholipases D (PLD) is involved in pathways both upstream and downstream of NOX-mediated ROS generation and identify NtPLD delta as a PLD isoform acting in the ROS response pathway. (c) 2012 Elsevier GmbH. All rights reserved.



FAU Authors / FAU Editors

Kost, Benedikt Prof. Dr.
Lehrstuhl für Zellbiologie


External institutions
Academy of Sciences of the Czech Republic (ASCR) / Akademie věd České republiky (AVČR)
Polska Akademia Nauk (PAN) / Polish Academy of Sciences
Spanish National Research Council / Consejo Superior de Investigaciones Científicas (CSIC)
Univerzita Karlova v Praze / Charles University in Prague


How to cite

APA:
Potocky, M., Pejchar, P., Gutkowska, M., Jose Jimenez-Quesada, M., Potocka, A., De Dios Alche, J.,... Zarsky, V. (2012). NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases. Journal of Plant Physiology, 169(16), 1654-1663. https://dx.doi.org/10.1016/j.jplph.2012.05.014

MLA:
Potocky, Martin, et al. "NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases." Journal of Plant Physiology 169.16 (2012): 1654-1663.

BibTeX: 

Last updated on 2018-08-08 at 13:53