Dietrich P (2002)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2002
Book Volume: 32
Pages Range: 623-630
Journal Issue: 4
DOI: 10.1046/j.1365-313X.2002.01451.x
Open Access Link: http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.2002.01451.x/abstract;jsessionid=CF1ECC7404A81CC2198266A8B8D6F826.f03t02
Stomata open in response to red and blue light. Red light-induced stomatal movement is mediated by guard cell chloroplasts and related to K+-uptake into these motor cells. We have combined a new type of microchlorophyll fluorometer with the patch-clamp technique for parallel studies of the photosynthetic electron transport and activity of plasma membrane K+ channels in single guard cell protoplast. In the whole-cell configuration and presence of ATP in the patch-pipette, the activity of the K+-uptake channels remained constant throughout the course of an experiment (up to 30 min) while photosynthetic activity declined to about 50%. In the absence of ATP inward K+ currents declined in a time-dependent manner. Under these ATP-free conditions, photosynthetic electron transport was completely blocked within 8 min. ADP together with orthophosphate was able to prevent inhibition of photosynthetic electron transport and run-down of K+-channel activity. The results demonstrate that the combination of these two techniques is suited to directly study cytosolic factors as common regulators of photosynthesis and plasma membrane transport within a single-cell.
APA:
Dietrich, P. (2002). Parallel recordings of photosynthetic electron transport and K+-channel activity in single guard cells. Plant Journal, 32(4), 623-630. https://dx.doi.org/10.1046/j.1365-313X.2002.01451.x
MLA:
Dietrich, Petra. "Parallel recordings of photosynthetic electron transport and K+-channel activity in single guard cells." Plant Journal 32.4 (2002): 623-630.
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