Maintenance of a bone collagen phenotype by osteoblast-like cells in 3D periodic porous titanium (Ti-6Al-4 V) structures fabricated by selective electron beam melting

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autor(en): Hrabe NW, Heinl P, Bordia RK, Körner C, Fernandes RJ
Zeitschrift: Connective Tissue Research
Jahr der Veröffentlichung: 2013
Band: 54
Heftnummer: 6
Seitenbereich: 351-360
ISSN: 0300-8207
Sprache: Englisch


Abstract


Regular 3D periodic porous Ti-6Al-4V structures were fabricated by the selective electron beam melting method (EBM) over a range of relative densities (0.17-0.40) and pore sizes (500-1500m). Structures were seeded with human osteoblast-like cells (SAOS-2) and cultured for four weeks. Cells multiplied within these structures and extracellular matrix collagen content increased. Type I and type V collagens typically synthesized by osteoblasts were deposited in the newly formed matrix with time in culture. High magnification scanning electron microscopy revealed cells attached to surfaces on the interior of the structures with an increasingly fibrous matrix. The in-vitro results demonstrate that the novel EBM-processed porous structures, designed to address the effect of stress-shielding, are conducive to osteoblast attachment, proliferation and deposition of a collagenous matrix characteristic of bone. © 2013 Informa Healthcare USA, Inc.



FAU-Autoren / FAU-Herausgeber

Heinl, Peter
Lehrstuhl für Werkstoffwissenschaften (Werkstoffkunde und Technologie der Metalle)
Körner, Carolin Prof. Dr.-Ing.
Lehrstuhl für Werkstoffwissenschaften (Werkstoffkunde und Technologie der Metalle)


Autor(en) der externen Einrichtung(en)
University of Washington


Zitierweisen

APA:
Hrabe, N.W., Heinl, P., Bordia, R.K., Körner, C., & Fernandes, R.J. (2013). Maintenance of a bone collagen phenotype by osteoblast-like cells in 3D periodic porous titanium (Ti-6Al-4 V) structures fabricated by selective electron beam melting. Connective Tissue Research, 54(6), 351-360. https://dx.doi.org/10.3109/03008207.2013.822864

MLA:
Hrabe, Nikolas W., et al. "Maintenance of a bone collagen phenotype by osteoblast-like cells in 3D periodic porous titanium (Ti-6Al-4 V) structures fabricated by selective electron beam melting." Connective Tissue Research 54.6 (2013): 351-360.

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Zuletzt aktualisiert 2018-06-12 um 20:50