Lee HH, Frasch M (2000)
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2000
Publisher: COMPANY OF BIOLOGISTS LTD
Book Volume: 127
Pages Range: 5497-5508
Journal Issue: 24
Inactivation of either the secreted protein Wingless (Wg) or the forkhead domain transcription factor Sloppy Paired (Slp) has been shown to produce similar effects in the developing Drosophila embryo. In the ectoderm, both gene products are required for the formation of the segmental portions marked by naked cuticle. In the mesoderm, Wg and Sip activities are crucial for the suppression of bagpipe (bap), and hence visceral mesoderm formation, and the promotion of somatic muscle and heart formation within the anterior portion of each parasegment, In this report, we show that, during these developmental processes, wg and sip act in a common pathway in which sip serves as a direct target of Wg signals that mediates Wg effects in both germ layers. We present evidence that the induction of sip by Wg involves binding of the Wg effector Pangolin (Drosophila Lef-1/TCF) to multiple binding sites within a Wg-responsive enhancer that is located in 5' flanking regions of the slp1 gene. Based upon our genetic and molecular analysis, we conclude that Wg signaling induces striped expression of Sip in the mesoderm, Mesodermal Sip is then sufficient to abrogate the induction of bagpipe by Dpp/Tinman, which explains the periodic arrangement of trunk visceral mesoderm primordia in wild type embryos. Conversely, mesodermal Sip is positively required, although not sufficient, for the specification of somatic muscle and heart progenitors. We propose that Wg-induced slp provides striped mesodermal domains with the competence to respond to subsequent sip-independent Wg signals that induce somatic muscle and heart progenitors. We also propose that in wg-expressing ectodermal cells, sip is an integral component in an autocrine feedback loop of Wg signaling.
APA:
Lee, H.-H., & Frasch, M. (2000). Wingless effects mesoderm patterning and ectoderm segmentation events via induction of its downstream target sloppy paired. Development, 127(24), 5497-5508.
MLA:
Lee, Hsiu-Hsiang, and Manfred Frasch. "Wingless effects mesoderm patterning and ectoderm segmentation events via induction of its downstream target sloppy paired." Development 127.24 (2000): 5497-5508.
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