Systems Analysis Reveals High Genetic and Antigen-Driven Predetermination of Antibody Repertoires throughout B Cell Development

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autor(en): Greiff V, Menzel U, Miho E, Weber C, Riedel R, Cook S, Valai A, Lopes T, Radbruch A, Winkler T, Reddy ST
Zeitschrift: Cell Reports
Verlag: Elsevier B.V.
Jahr der Veröffentlichung: 2017
Band: 19
Heftnummer: 7
Seitenbereich: 1467-1478
ISSN: 2211-1247


Abstract


Antibody repertoire diversity and plasticity is crucial for broad protective immunity. Repertoires change in size and diversity across multiple B cell developmental stages and in response to antigen exposure. However, we still lack fundamental quantitative understanding of the extent to which repertoire diversity is predetermined. Therefore, we implemented a systems immunology framework for quantifying repertoire predetermination on three distinct levels: (1) B cell development (pre-B cell, naive B cell, plasma cell), (2) antigen exposure (three structurally different proteins), and (3) four antibody repertoire components (V-gene usage, clonal expansion, clonal diversity, repertoire size) extracted from antibody repertoire sequencing data (400 million reads). Across all three levels, we detected a dynamic balance of high genetic (e.g., >90% for V-gene usage and clonal expansion in naive B cells) and antigen-driven (e.g., 40% for clonal diversity in plasma cells) predetermination and stochastic variation. Our study has implications for the prediction and manipulation of humoral immunity.



FAU-Autoren / FAU-Herausgeber

Winkler, Thomas Prof. Dr.
Professur für Genetik


Autor(en) der externen Einrichtung(en)
Deutsches Rheuma-Forschungszentrum (DRFZ)
École Polytechnique Fédérale de Lausanne (EPFL)
Shire Austria GmbH


Zitierweisen

APA:
Greiff, V., Menzel, U., Miho, E., Weber, C., Riedel, R., Cook, S.,... Reddy, S.T. (2017). Systems Analysis Reveals High Genetic and Antigen-Driven Predetermination of Antibody Repertoires throughout B Cell Development. Cell Reports, 19(7), 1467-1478. https://dx.doi.org/10.1016/j.celrep.2017.04.054

MLA:
Greiff, Victor, et al. "Systems Analysis Reveals High Genetic and Antigen-Driven Predetermination of Antibody Repertoires throughout B Cell Development." Cell Reports 19.7 (2017): 1467-1478.

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Zuletzt aktualisiert 2018-16-10 um 07:02