Arabidopsis POLYOL TRANSPORTER5, a new member of the monosaccharide transporter-like superfamily, mediates H+-Symport of numerous substrates, including myo-inositol, glycerol, and ribose.

Klepek Y, Geiger D, Stadler R, Klebl F, Landouar-Arsivaud L, Lemoine R, Hedrich R, Sauer N (2005)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2005

Journal

The Plant Cell American Society of Plant Biologists

Book Volume: 17

Pages Range: 204-218

Journal Issue: 1

DOI: 10.1105/tpc.104.026641

Open Access Link: http://www.plantcell.org/content/17/1/204.long

Abstract

Six genes of the Arabidopsis thaliana monosaccharide transporter-like (MST-like) superfamily share significant homology with polyol transporter genes previously identified in plants translocating polyols (mannitol or sorbitol) in their phloem (celery [Apium graveolens], common plantain [Plantago major], or sour cherry [Prunus cerasus]). The physiological role and the functional properties of this group of proteins were unclear in Arabidopsis, which translocates sucrose and small amounts of raffinose rather than polyols. Here, we describe POLYOL TRANSPORTER5 (AtPLT5), the first member of this subgroup of Arabidopsis MST-like transporters. Transient expression of an AtPLT5-green fluorescent protein fusion in plant cells and functional analyses of the AtPLT5 protein in yeast and Xenopus oocytes demonstrate that AtPLT5 is located in the plasma membrane and characterize this protein as a broad-spectrum H+-symporter for linear polyols, such as sorbitol, xylitol, erythritol, or glycerol. Unexpectedly, however, AtPLT5 catalyzes also the transport of the cyclic polyol myo-inositol and of different hexoses and pentoses, including ribose, a sugar that is not transported by any of the previously characterized plant sugar transporters. RT-PCR analyses and AtPLT5 promoter-reporter gene plants revealed that AtPLT5 is most strongly expressed in Arabidopsis roots, but also in the vascular tissue of leaves and in specific floral organs. The potential physiological role of AtPLT5 is discussed.

Authors with CRIS profile

Ruth Stadler Lehrstuhl für Molekulare Pflanzenphysiologie Franz Klebl Lehrstuhl für Molekulare Pflanzenphysiologie Norbert Sauer Lehrstuhl für Molekulare Pflanzenphysiologie

Involved external institutions

National Center for Scientific Research / Centre national de la recherche scientifique (CNRS) FR France (FR) Julius-Maximilians-Universität Würzburg DE Germany (DE)

How to cite

APA:

Klepek, Y., Geiger, D., Stadler, R., Klebl, F., Landouar-Arsivaud, L., Lemoine, R.,... Sauer, N. (2005). Arabidopsis POLYOL TRANSPORTER5, a new member of the monosaccharide transporter-like superfamily, mediates H+-Symport of numerous substrates, including myo-inositol, glycerol, and ribose. The Plant Cell, 17(1), 204-218. https://dx.doi.org/10.1105/tpc.104.026641

MLA:

Klepek, Yvonne, et al. "Arabidopsis POLYOL TRANSPORTER5, a new member of the monosaccharide transporter-like superfamily, mediates H+-Symport of numerous substrates, including myo-inositol, glycerol, and ribose." The Plant Cell 17.1 (2005): 204-218.

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