Behavior of encapsulated MG-63 cells in RGD and gelatine-modified alginate hydrogels

Grigore A, Sarker B, Fabry B, Boccaccini AR, Detsch R (2014)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2014

Journal

Tissue Engineering - Part A Mary Ann Liebert

Book Volume: 20

Pages Range: 2140-2150

DOI: 10.1089/ten.tea.2013.0416

Abstract

Achieving cell spreading and proliferation inside hydrogels that are compatible with microencapsulation technology represents a major challenge for tissue engineering scaffolding and for the development of three-dimensional cell culture models. In this study, microcapsules of 650-900μm in diameter were fabricated from oxidized alginate covalently cross-linked with gelatine (AlGel). Schiff's base bond formed in AlGel, detected by Fourier transform infrared spectroscopy, which confirmed the cross-linking of oxidized alginate with gelatine. Biological properties of alginate based hydrogels were studied by comparing the viability and morphology of MG-63 osteosarcoma cells encapsulated in gelatine and RGD-modified alginate. We hypothesized that the presence of gelatine and RGD will support cell adhesion and spreading inside the microcapsules and finally, also vascular endothelial growth factor (VEGF) secretion. After 4 days of incubation, cells formed extensive cortical protrusions and after 2 weeks they proliferated, migrated, and formed cellular networks through the AlGel material. In contrast, cells encapsulated in pure alginate and in RGD-modified alginate formed spherical aggregates with limited cell mobility and VEGF secretion. Metabolic activity was doubled after 5 days of incubation, making AlGel a promising material for cell encapsulation. © Mary Ann Liebert, Inc.

Authors with CRIS profile

Alexandra Grigore Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Bapi Sarker Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Ben Fabry Lehrstuhl für Biophysik Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Rainer Detsch Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

How to cite

APA:

Grigore, A., Sarker, B., Fabry, B., Boccaccini, A.R., & Detsch, R. (2014). Behavior of encapsulated MG-63 cells in RGD and gelatine-modified alginate hydrogels. Tissue Engineering - Part A, 20, 2140-2150. https://dx.doi.org/10.1089/ten.tea.2013.0416

MLA:

Grigore, Alexandra, et al. "Behavior of encapsulated MG-63 cells in RGD and gelatine-modified alginate hydrogels." Tissue Engineering - Part A 20 (2014): 2140-2150.

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