The corepressor activity of Alien is controlled by CREB-binding protein/p300

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Eckey M, Kraft F, Kob R, Escher N, Asim M, Fischer H, Fritsche MK, Melle C, Baniahmad A
Zeitschrift: Febs Journal
Verlag: Wiley-Blackwell
Jahr der Veröffentlichung: 2013
Band: 280
Heftnummer: 8
Seitenbereich: 1861-1868
ISSN: 1742-464X


Abstract


The regulation of gene repression by corepressors is a controlled process. Surface-enhanced laser desorption ionization MS proteomic analysis and a yeast two-hybrid screen showed independently that the corepressor Alien interacts with the CREB-binding protein (CBP) coactivator. This interaction was further confirmed by coimmunoprecipitation and glutathione S-transferase pull-down experiments, suggesting that Alien interacts invivo and invitro with the histone acetyltransferase (HAT) coactivators CBP and its paralog p300. Acetylation detection experiments indicated that Alien is acetylated invivo. Furthermore, Alien interacts with the central region of CBP/p300 containing the HAT domain and becomes acetylated invitro. When an inhibitor of CBP/p300 HAT activity was employed, the Alien-mediated silencing was enhanced. Thus, these findings suggest crosstalk between corepressors and coactivators, and indicate fine-tuning of corepressor function by post-translational modification through corepressor acetylation.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Kob, Robert Dr.
Professur für Innere Medizin - Geriatrie


Einrichtungen weiterer Autorinnen und Autoren

Universitätsklinikum Jena


Zitierweisen

APA:
Eckey, M., Kraft, F., Kob, R., Escher, N., Asim, M., Fischer, H.,... Baniahmad, A. (2013). The corepressor activity of Alien is controlled by CREB-binding protein/p300. Febs Journal, 280(8), 1861-1868. https://dx.doi.org/10.1111/febs.12211

MLA:
Eckey, Maren, et al. "The corepressor activity of Alien is controlled by CREB-binding protein/p300." Febs Journal 280.8 (2013): 1861-1868.

BibTeX: 

Zuletzt aktualisiert 2018-10-08 um 05:42