The corepressor activity of Alien is controlled by CREB-binding protein/p300
Eckey M, Kraft F, Kob R, Escher N, Asim M, Fischer H, Fritsche MK, Melle C, Baniahmad A (2013)
Publication Status: Published
Publication Type: Journal article
Publication year: 2013
Journal
Publisher: Wiley-Blackwell
Book Volume: 280
Pages Range: 1861-1868
Journal Issue: 8
DOI: 10.1111/febs.12211
Abstract
The regulation of gene repression by corepressors is a controlled process. Surface-enhanced laser desorption ionization MS proteomic analysis and a yeast two-hybrid screen showed independently that the corepressor Alien interacts with the CREB-binding protein (CBP) coactivator. This interaction was further confirmed by coimmunoprecipitation and glutathione S-transferase pull-down experiments, suggesting that Alien interacts invivo and invitro with the histone acetyltransferase (HAT) coactivators CBP and its paralog p300. Acetylation detection experiments indicated that Alien is acetylated invivo. Furthermore, Alien interacts with the central region of CBP/p300 containing the HAT domain and becomes acetylated invitro. When an inhibitor of CBP/p300 HAT activity was employed, the Alien-mediated silencing was enhanced. Thus, these findings suggest crosstalk between corepressors and coactivators, and indicate fine-tuning of corepressor function by post-translational modification through corepressor acetylation.
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Germany (DE)How to cite
APA:
Eckey, M., Kraft, F., Kob, R., Escher, N., Asim, M., Fischer, H.,... Baniahmad, A. (2013). The corepressor activity of Alien is controlled by CREB-binding protein/p300. Febs Journal, 280(8), 1861-1868. https://dx.doi.org/10.1111/febs.12211
MLA:
Eckey, Maren, et al. "The corepressor activity of Alien is controlled by CREB-binding protein/p300." Febs Journal 280.8 (2013): 1861-1868.
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