Enhancement and induction of HIV-1 infection through an assembled peptide derived from the CD4 binding site of gp120

Groß A, Roedel K, Kneidl B, Donhauser N, Mössl M, Lump E, Muench J, Schmidt B, Eichler J (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Book Volume: 16

Pages Range: 446-54

Journal Issue: 3

DOI: 10.1002/cbic.201402545

Abstract

Contact between the human immunodeficiency virus (HIV-1) and its target cell is initiated by the interaction of viral gp120 with cellular CD4. An assembled peptide (CD4bs-M) that presents the CD4 binding site of gp120 was previously shown to inhibit the gp120-CD4 interaction. Here, we demonstrate that CD4bs-M selectively enhances infection of cells with HIV-1, whereas infection with herpes simplex virus remains largely unaffected. The effects of CD4bs-M variants containing D-amino acids, or prolines at selected positions, point to the importance of side chain orientation and spatial orientation of this fragment. Furthermore, CD4bs-M was shown to assemble into amyloid-like fibrils that capture HIV-1 particles, which likely contributes to the infection-enhancing effect. Beyond infection enhancement, CD4bs-M enabled HIV-1 infection of CD4-negative cells, suggesting that binding of the peptide to gp120 facilitates interaction of gp120 with coreceptors, which might in turn enhance HIV-1 entry.

Authors with CRIS profile

Involved external institutions

Universität Regensburg Germany (DE) Universität Ulm Germany (DE)

How to cite

APA:

Groß, A., Roedel, K., Kneidl, B., Donhauser, N., Mössl, M., Lump, E.,... Eichler, J. (2015). Enhancement and induction of HIV-1 infection through an assembled peptide derived from the CD4 binding site of gp120. ChemBioChem, 16(3), 446-54. https://doi.org/10.1002/cbic.201402545

MLA:

Groß, Andrea, et al. "Enhancement and induction of HIV-1 infection through an assembled peptide derived from the CD4 binding site of gp120." ChemBioChem 16.3 (2015): 446-54.

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