Spinach ferredoxin is a calcium-binding protein

Journal article

Publication Details

Author(s): Surek B, Kreimer G, Melkonian M, Latzko E
Journal: Planta
Publisher: Springer Verlag (Germany)
Publication year: 1987
Volume: 171
Journal issue: 4
Pages range: 565-568
ISSN: 0032-0935


Spinach-leaf ferredoxin was identified as a calcium-binding protein by 45Ca autoradiography on nitrocellulose membranes and with the cationic carbocyanine dye 1-ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl] naphtho[1,2-d]thiazolium bromide (“stains-all”). Binding of 45Ca was observed at pH 6.8 and pH 7.8 and in the presence of 5 mM and 20 mM MgCl2. At the higher MgCl2 concentration the Ca2+-binding capacity is reduced. Only micromolar concentrations of LaCl3, however, are required to achieve a similar effect. Both the oxidized and reduced forms of ferredoxin bind calcium.

External institutions
Universität zu Köln
Westfälische Wilhelms-Universität (WWU) Münster

How to cite

Surek, B., Kreimer, G., Melkonian, M., & Latzko, E. (1987). Spinach ferredoxin is a calcium-binding protein. Planta, 171(4), 565-568. https://dx.doi.org/10.1007/BF00392307

Surek, Barbara, et al. "Spinach ferredoxin is a calcium-binding protein." Planta 171.4 (1987): 565-568.


Last updated on 2018-08-07 at 23:10