Effects of a strongly adsorbed additive on process performance in chiral preparative chromatography

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autorinnen und Autoren: Forssén P, Arnell R, Kaspereit M, Seidel-Morgenstern A, Fornstedt T
Zeitschrift: Journal of Chromatography A
Verlag: Elsevier
Jahr der Veröffentlichung: 2008
Band: 1212
Seitenbereich: 89-97
ISSN: 0021-9673
Sprache: Englisch


Abstract


The shapes of elution profiles are often significantly influenced by the presence of strongly adsorbed additives in the mobile phase. This aspect needs to be considered in quantitative optimization of preparative chromatography. The theoretical study carried out here is based on available thermodynamic information for the enantiomers of three β-blockers, alprenolol, propranolol, and atenolol, on a teicoplanin chiral stationary phase (Chirobiotic T) using methanol/acetonitrile as the mobile phase and acetic acid/triethylamine as the additive. The properties of this strong additive made it possible to tune the binary elution profiles in any combination of the following apparent band shapes: anti-Langmuir/anti-Langmuir, anti-Langmuir/Langmuir and Langmuir/Langmuir. Optimization of the productivity and yield, when performing repetitive batch injections, was investigated using the equilibrium dispersive model. We show that it is important to consider the invisible additive perturbation peak when defining the cycle time and therefore a model-based optimization needs to take this into account. Furthermore, both productivity and yield could be improved for the two unusual shape combinations in comparison to the traditional Langmuir/Langmuir case. © 2008 Elsevier B.V. All rights reserved.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Kaspereit, Malte Prof. Dr.-Ing.
Professur für Thermische Verfahrenstechnik


Zitierweisen

APA:
Forssén, P., Arnell, R., Kaspereit, M., Seidel-Morgenstern, A., & Fornstedt, T. (2008). Effects of a strongly adsorbed additive on process performance in chiral preparative chromatography. Journal of Chromatography A, 1212, 89-97. https://dx.doi.org/10.1016/j.chroma.2008.10.040

MLA:
Forssén, Patrik, et al. "Effects of a strongly adsorbed additive on process performance in chiral preparative chromatography." Journal of Chromatography A 1212 (2008): 89-97.

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