Amino acids as possible alternative nitrogen source for growth of Euglena gracilis Z in life support systems

Journal article
(Original article)


Publication Details

Author(s): Richter P, Liu Y, An Y, Li X, Nasir A, Strauch S, Becker I, Krüger J, Schuster M, Ntefidou M, Daiker V, Haag F, Aiach A, Lebert M
Journal: Life Sciences in Space Research
Publication year: 2015
Volume: 4
Pages range: 1-5
ISSN: 2214-5532


Abstract


In recent times Euglena gracilis Z was employed as primary producer in closed environmental life-support system (CELSS), e.g. in space research. The photosynthetic unicellular flagellate is not capable of utilizing nitrate, nitrite, and urea as nitrogen source. Therefore, ammonium is supplied as an N-source in the lab (provided as diammonium-dihydrogenphosphate, (NH)HPO) to E. gracilis cultures. While nitrate exerts low toxicity to organisms, ammonium is harmful for many aquatic organisms especially, at high pH-values, which causes the ionic NH4+ (low toxicity) to be partially transformed into the highly toxic ammonia, NH. In earlier reports, Euglena gracilis was described to grow with various amino acids as sole N-source. Our aim was to investigate alternatives for (NH)HPO as N-source with lower toxicity for organisms co-cultivated with Euglena in a CELSS. The growth kinetics of Euglena gracilis cultures was determined in the presence of different amino acids (glycine, glutamine, glutamic acid, leucine, and threonine). In addition, uptake of those amino acids by the cells was measured. Cell growth in the presence of glycine and glutamine was quite comparable to the growth in (NH)HPO containing cultures while a delay in growth was observed in the presence of leucine and threonine. Unlike, aforementioned amino acids glutamate consumption was very poor. Cell density and glutamate concentration were almost unaltered throughout the experiment and the culture reached the stationary phase within 8 days. The data are compared with earlier studies in which utilization of amino acids in Euglena gracilis was investigated. All tested amino acids (glutamate with limitations) were found to have the potential of being an alternative N-source for Euglena gracilis. Hence, these amino acids can be used as a non-toxic surrogate for (NH)HPO.



FAU Authors / FAU Editors

Becker, Ina
Lehrstuhl für Zellbiologie
Daiker, Viktor Dr.
Lehrstuhl für Zellbiologie
Krüger, Julia
Lehrstuhl für Zellbiologie
Lebert, Michael PD Dr.
Lehrstuhl für Zellbiologie
Nasir, Adeel Dr.
Lehrstuhl für Zellbiologie
Ntefidou, Maria Dr.
Lehrstuhl für Zellbiologie
Richter, Peter Dr.
Lehrstuhl für Zellbiologie
Schuster, Martin
Lehrstuhl für Zellbiologie
Strauch, Sebastian Dr.
Lehrstuhl für Zellbiologie


External institutions
Al-Andalus University for Medical Sciences / جامعة الأندلس الخاصة للعلوم الطبية‎‎
Institute of Hydrobiology, Chinese Academy of Sciences (CAS)


How to cite

APA:
Richter, P., Liu, Y., An, Y., Li, X., Nasir, A., Strauch, S.,... Lebert, M. (2015). Amino acids as possible alternative nitrogen source for growth of Euglena gracilis Z in life support systems. Life Sciences in Space Research, 4, 1-5. https://dx.doi.org/10.1016/j.lssr.2014.11.001

MLA:
Richter, Peter, et al. "Amino acids as possible alternative nitrogen source for growth of Euglena gracilis Z in life support systems." Life Sciences in Space Research 4 (2015): 1-5.

BibTeX: 

Last updated on 2018-24-11 at 20:50