ZIP3, a New Splice Variant of the PKC-ζ-interacting Protein Family, Binds to GABAC Receptors, PKC-ζ, and Kvβ2
Croci C, Brandstätter JH, Enz R (2003)
Publication Type: Journal article
Publication year: 2003
Journal
Publisher: American Society for Biochemistry and Molecular Biology
Pages Range: 6128-6135
Journal Issue: 278
DOI: 10.1074/jbc.M205162200
Abstract
The correct targeting of modifying enzymes to ion channels and neurotransmitter receptors represents an important biological mechanism to control neuronal excitability. The recent cloning of protein kinase C-zeta interacting proteins (ZIP1, ZIP2) identified new scaffolds linking the atypical protein kinase PKC-ζ to target proteins. GABAC receptors are composed of three ρ subunits (ρ1-3) that are highly expressed in the retina, where they are clustered at synaptic terminals of bipolar cells. A yeast two-hybrid screen for the GABAC receptor ρ3 subunit identified ZIP3, a new C-terminal splice variant of the ZIP protein family. ZIP3 was ubiquitously expressed in non-neuronal and neuronal tissues, including the retina. The ρ3-binding region of ZIP3 contained a ZZ-zinc finger domain, which interacted with 10 amino acids conserved in ρ1-3 but not in GABAA receptors. Consistently, only ρ1-3 subunits bound to ZIP3. ZIP3 formed dimers with ZIP1-3 and interacted with PKC-ζ and the shaker-type potassium channel subunit Kvβ2. Different domains of ZIP3 interacted with PKC-ζ and the p3 subunit, and simultaneous assembly of ZIP3, PKC-ζ and ρ3 was demonstrated in vitro. Subcellular co-expression of ZIP3 binding partners in the retina supported the proposed protein interactions. Our results indicate the formation of a ternary postsynaptic complex containing PKC-ζ, ZIP3, and GABAC receptors.
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How to cite
APA:
Croci, C., Brandstätter, J.H., & Enz, R. (2003). ZIP3, a New Splice Variant of the PKC-ζ-interacting Protein Family, Binds to GABAC Receptors, PKC-ζ, and Kvβ2. Journal of Biological Chemistry, 278, 6128-6135. https://dx.doi.org/10.1074/jbc.M205162200
MLA:
Croci, Cristina, Johann Helmut Brandstätter, and Ralf Enz. "ZIP3, a New Splice Variant of the PKC-ζ-interacting Protein Family, Binds to GABAC Receptors, PKC-ζ, and Kvβ2." Journal of Biological Chemistry 278 (2003): 6128-6135.
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