Proteomic Response of Human Umbilical Vein Endothelial Cells to Histamine Stimulation

Emirbayer PE, Sinha A, Ignatchenko V, Hoyer S, Dörrie J, Schaft N, Pischetsrieder M, Kislinger T (2017)

Publication Status: Published

Publication Type: Journal article

Publication year: 2017

Journal

Proteomics Wiley-VCH Verlag

Publisher: WILEY

Book Volume: 17

Article Number: 1700116

Journal Issue: 21

DOI: 10.1002/pmic.201700116

Abstract

The histamine receptors (HRs) represent a subclass of G protein-coupled receptors (GPCRs) and comprise four subtypes. Due to their numerous physiological and pathological effects, HRs are popular drug targets for the treatment of allergic reactions or the regulation of gastric acid secretion. Hence, an understanding of the functional selectivity of HR ligands has gained importance. These ligands can bind to specific GPCRs and selectively activate defined pathways. Supporting the activation of a therapeutically necessary pathway without the activation of other signaling cascades can result in drugs with more specific activity and fewer side effects. To evaluate the cellular consequences resulting from receptor binding, comprehensive analyses of cellular protein alterations upon incubation with ligands are required. For this purpose, endothelial cells are treated with histamine, as the endogenous ligand of HRs, to obtain a global overview of its cellular effects. Quantitative proteomics and pathway analyses of histamine-treated and untreated cells reveal enrichment of the nuclear factor-B and tumor necrosis factor signaling pathways, cytokine-cytokine receptor interactions, complement and coagulation cascades, and acute inflammatory processes upon histamine treatment. This strategy offers the opportunity to monitor HR-mediated signaling in a multidimensional manner.

Authors with CRIS profile

Pelin Esma Emirbayer Graduiertenkolleg 1910/3 Medizinische Chemie selektiver GPCR-Liganden Jan Dörrie Department of Dermatology Niels Schaft Department of Dermatology Monika Pischetsrieder Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)

Involved external institutions

University Health Network (UHN) CA Canada (CA)

How to cite

APA:

Emirbayer, P.E., Sinha, A., Ignatchenko, V., Hoyer, S., Dörrie, J., Schaft, N.,... Kislinger, T. (2017). Proteomic Response of Human Umbilical Vein Endothelial Cells to Histamine Stimulation. Proteomics, 17(21). https://dx.doi.org/10.1002/pmic.201700116

MLA:

Emirbayer, Pelin Esma, et al. "Proteomic Response of Human Umbilical Vein Endothelial Cells to Histamine Stimulation." Proteomics 17.21 (2017).

BibTeX: Download