Calix[4]arenes as molecular platforms for magnetic resonance imaging (MRI) contrast agents

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autor(en): Schatz J
Zeitschrift: Chemistry - A European Journal
Verlag: Wiley-VCH Verlag
Jahr der Veröffentlichung: 2009
Band: 15
Heftnummer: 13
Seitenbereich: 3290-3296
ISSN: 0947-6539


Abstract


An amphiphilic conjugate 1 of a calix[4]arene with four Gd-DOTA chelates (DOTA = 1,4,7,10-tetra(carboxymethyl)-1,4,7,10-tetraazacyclododecane) was prepared and the properties relevant to its application as a magnetic resonance imaging (MRI) contrast agent were investigated by NMR, dynamic light scattering (DLS), and cryo-electron microscopy (cryo-TEM). The compound aggregates in water; its critical micelle concentration (cmc) is 0.21 mM (or 0.84mM for Gd) at 37°C. The relaxivity of the aggregates at 37°C and 20MHz (18.3s per mM Gd or 73.2 s per mM 1) is about twice that of the monomer. Nuclear magnetic relaxation dispersion (NMRD) profiles show the relaxivity of the monomer to be almost independent of the magnetic field strength up to 60 MHz. At higher concentrations, the NMRD profiles exhibit a maximum at about 20MHz, which is typical for high molecular volumes. The average water residence lifetime is 1.20 μs at 298 K as determined by O NMR. The rotational correlation time of the monomer (390 ns at 37°C) is very close to the optimal value predicted for high-field contrast agents. Monomer as well as micelles are very rigid systems with negligible local contributions to the overall rotational dynamics. The binding to human serum albumin (HSA) is significant (K= 1.2 × 10 M ) and the relaxivity of the HSA adduct at 20 MHz is 24.6 s per mM Gd or 98.5 s per mM 1. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.



FAU-Autoren / FAU-Herausgeber

Schatz, Jürgen Prof. Dr.
Professur für Organische Chemie

Zuletzt aktualisiert 2018-11-08 um 03:14