Org-1, the Drosophila ortholog of Tbx1, is a direct activator of known identity genes during muscle specification

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autorinnen und Autoren: Schaub C, Nagaso H, Jin H, Frasch M
Zeitschrift: Development
Verlag: COMPANY OF BIOLOGISTS LTD
Jahr der Veröffentlichung: 2012
Band: 139
Heftnummer: 5
Seitenbereich: 1001-1012
ISSN: 0950-1991
eISSN: 1477-9129


Abstract


Members of the T-Box gene family of transcription factors are important players in regulatory circuits that generate myogenic and cardiogenic lineage diversities in vertebrates. We show that during somatic myogenesis in Drosophila, the single ortholog of vertebrate Tbx1, optomotor-blind-related-gene-1 (org-1), is expressed in a small subset of muscle progenitors, founder cells and adult muscle precursors, where it overlaps with the products of the muscle identity genes ladybird (lb) and slouch (slou). In addition, org-1 is expressed in the lineage of the heart-associated alary muscles. org-1 null mutant embryos lack Lb and Slou expression within the muscle lineages that normally co-express org-1. As a consequence, the respective muscle fibers and adult muscle precursors are either severely malformed or missing, as are the alary muscles. To address the mechanisms that mediate these regulatory interactions between Org-1, Lb and Slou, we characterized distinct enhancers associated with somatic muscle expression of lb and slou. We demonstrate that these lineage-and stage-specific cis-regulatory modules (CRMs) bind Org-1 in vivo, respond to org-1 genetically and require T-box domain binding sites for their activation. In summary, we propose that org-1 is a common and direct upstream regulator of slou and lb in the developmental pathway of these two neighboring muscle lineages. Cross-repression between slou and lb and combinatorial activation of lineage-specific targets by Org-1-Slou and Org-1- Lb, respectively, then leads to the distinction between the two lineages. These findings provide new insights into the regulatory circuits that control the proper pattering of the larval somatic musculature in Drosophila.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Frasch, Manfred Prof. Dr.
Lehrstuhl für Entwicklungsbiologie
Jin, Hong
Lehrstuhl für Entwicklungsbiologie
Schaub, Christoph Dr.
Lehrstuhl für Entwicklungsbiologie


Einrichtungen weiterer Autorinnen und Autoren

Icahn School of Medicine at Mount Sinai


Zitierweisen

APA:
Schaub, C., Nagaso, H., Jin, H., & Frasch, M. (2012). Org-1, the Drosophila ortholog of Tbx1, is a direct activator of known identity genes during muscle specification. Development, 139(5), 1001-1012. https://dx.doi.org/10.1242/dev.073890

MLA:
Schaub, Christoph, et al. "Org-1, the Drosophila ortholog of Tbx1, is a direct activator of known identity genes during muscle specification." Development 139.5 (2012): 1001-1012.

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