Effects of Cold Atmospheric Plasma (CAP) on ß-Defensins, Inflammatory Cytokines, and Apoptosis-Related Molecules in Keratinocytes In Vitro and In Vivo
Arndt S, Landthaler M, Zimmermann JL, Unger P, Wacker E, Shimizu T, Li YF, Morfill GE, Boßerhoff AK, Karrer S (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 10
Pages Range: e0120041
Journal Issue: 3
DOI: 10.1371/journal.pone.0120041
Abstract
Cold atmospheric plasma (CAP) has been gaining increasing interest as a new approach for the treatment of skin diseases or wounds. Although this approach has demonstrated promising antibacterial activity, its exact mechanism of action remains unclear. This study explored in vitro and in vivo whether CAP influences gene expression and molecular mechanisms in keratinocytes. Our results revealed that a 2 min CAP treatment using the MicroPlaSter ß in analogy to the performed clinical studies for wound treatment induces expression of IL-8, TGF-ß1, and TGF-ß2. In vitro and in vivo assays indicated that keratinocyte proliferation, migration, and apoptotic mechanisms were not affected by the CAP treatment under the applied conditions. Further, we observed that antimicrobial peptides of the ß-defensin family are upregulated after CAP treatment. In summary, our results suggest that a 2 min application of CAP induces gene expression of key regulators important for inflammation and wound healing without causing proliferation, migration or cell death in keratinocytes. The induction of ß-defensins in keratinocytes describes an absolutely new plasma strategy. Activation of antimicrobial peptides supports the well-known antibacterial effect of CAP treatment, whereas the mechanism of ß-defensin activation by CAP is not investigated so far.
Authors with CRIS profile
Involved external institutions
Universität Regensburg
Germany (DE)
Universitätsklinikum Regensburg
Germany (DE)
Max-Planck-Institut für extraterrestrische Physik (MPE) / Max Planck Institute for Extraterrestrial Physics
Germany (DE)
Germany (DE)
Universitätsklinikum Regensburg
Germany (DE)
Max-Planck-Institut für extraterrestrische Physik (MPE) / Max Planck Institute for Extraterrestrial Physics
Germany (DE)
How to cite
APA:
Arndt, S., Landthaler, M., Zimmermann, J.L., Unger, P., Wacker, E., Shimizu, T.,... Karrer, S. (2015). Effects of Cold Atmospheric Plasma (CAP) on ß-Defensins, Inflammatory Cytokines, and Apoptosis-Related Molecules in Keratinocytes In Vitro and In Vivo. PLoS ONE, 10(3), e0120041. https://doi.org/10.1371/journal.pone.0120041
MLA:
Arndt, Stephanie, et al. "Effects of Cold Atmospheric Plasma (CAP) on ß-Defensins, Inflammatory Cytokines, and Apoptosis-Related Molecules in Keratinocytes In Vitro and In Vivo." PLoS ONE 10.3 (2015): e0120041.
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