Endothelin-1 increases 2-arachidonoyl glycerol (2-AG) production in astrocytes.

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Details zur Publikation

Autor(en): Walter L
Zeitschrift: Glia
Jahr der Veröffentlichung: 2003
Band: 44
Heftnummer: 1
Seitenbereich: 85-90
ISSN: 0894-1491


Abstract


Astrocytes play an important role in neuroprotective responses. Recent studies indicate that endothelin-1, a neuropeptide upregulated during brain injury, increases levels of the endocannabinoid anandamide, a lipid with neuroprotective properties, in astrocytes in primary cultures. However, whether this neuropeptide also alters levels of 2-arachidonoyl glycerol (2-AG), the most abundant endocannabinoid in the CNS, in astrocytes remains unknown. In addition, 2-AG levels in astrocytes have never been measured. In this report we use chemical ionization gas chromatography/mass spectrometry to quantify picomole amounts of 2-AG in primary cultures of mouse astrocytes. We also demonstrate that endothelin-1 increases 2-AG production by 5-fold in these cells, a response that requires extracellular calcium and endothelin-1(A) receptor engagement. Immunocytochemistry showed that although cultured mouse neurons and microglia express cannabinoid receptors, cultured astrocytes do not. The data suggest that endothelin-1 modulates 2-AG production in astrocytes and that this endocannabinoid may participate in paracrine signaling toward neurons and microglia.



FAU-Autoren / FAU-Herausgeber

Walter, Lisa Dr.
Lehrstuhl für Informatik 14 (Maschinelles Lernen und Datenanalytik)

Zuletzt aktualisiert 2018-23-09 um 18:10