A quantitative hypermorphic CNGC allele confers ectopic calcium flux and impairs cellular development.

Chiasson DM, Haage K, Sollweck K, Brachmann A, Dietrich P, Parniske M (2017)


Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2017

Journal

Book Volume: 6

DOI: 10.7554/eLife.25012

Abstract

The coordinated control of Ca2+ signaling is essential for development in eukaryotes. Cyclic nucleotide-gated channel (CNGC) family members mediate Ca2+ influx from cellular stores in plants (Charpentier et al., 2016; Gao et al., 2016; Frietsch et al., 2007; Urquhart et al., 2007). Here, we report the unusual genetic behavior of a quantitative gain-of-function CNGC mutation (brush) in Lotus japonicus resulting in a leaky tetrameric channel. brush resides in a cluster of redundant CNGCs encoding subunits which resemble metazoan voltage-gated potassium (Kv1-Kv4) channels in assembly and gating properties. The recessive mongenic brush mutation impaired root development and infection by nitrogen-fixing rhizobia. The brush allele exhibited quantitative behavior since overexpression of the cluster subunits was required to suppress the brush phenotype. The results reveal a mechanism by which quantitative competition between channel subunits for tetramer assembly can impact the phenotype of the mutation carrier.

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How to cite

APA:

Chiasson, D.M., Haage, K., Sollweck, K., Brachmann, A., Dietrich, P., & Parniske, M. (2017). A quantitative hypermorphic CNGC allele confers ectopic calcium flux and impairs cellular development. eLife, 6. https://dx.doi.org/10.7554/eLife.25012

MLA:

Chiasson, David M, et al. "A quantitative hypermorphic CNGC allele confers ectopic calcium flux and impairs cellular development." eLife 6 (2017).

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