A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Gebrekidan MT, Knipfer C, Stelzle F, Popp J, Will S, Bräuer A
Zeitschrift: Journal of Raman Spectroscopy
Verlag: Wiley-Blackwell
Jahr der Veröffentlichung: 2016
Band: 47
Heftnummer: 2
Seitenbereich: 198-209
ISSN: 0377-0486
Sprache: Englisch


Abstract


A biochemical characterization of pathologies in biological tissue can be provided by Raman spectroscopy. Often, the raw spectrum is severely affected by fluorescence interference. We report and compare various spectra-processing approaches required for the purification of Raman spectra from heavily fluorescence-interfered raw spectra according to the shifted-excitation Raman difference spectroscopy method. These approaches cover the entire spectra-processing chain from the raw spectra to the purified Raman spectra. In detail, we compared (1) area normalization versus z-score normalization, (2) direct reconstruction of the difference spectra versus reconstruction of zero-centered difference spectra and (3) collective baseline correction of the reconstructed spectra versus piecewise baseline correction of the reconstructed spectra and, finally, (4) analyzed the influence of the shift of the excitation wavelength on the quality of the reconstructed spectra. Statistical analysis of the spectra showed that - in our experiments - the best results were obtained for the z-score normalization before subtraction of the normalized spectra, followed by zero-centering of the difference spectra before reconstruction and a piecewise baseline correction of the pure Raman spectra. With our equipment, a wavelength shift from 784 to 785nm provided reconstructed spectra of best quality. The analyzed specimens were different tissue types of pigs, tissue from the oral cavity of humans and a model solution of dye dissolved in ethanol. (c) 2015 The Authors. Journal of Raman Spectroscopy published by John Wiley & Sons Ltd.



FAU-Autoren / FAU-Herausgeber

Bräuer, Andreas PD Dr.
Erlangen Graduate School in Advanced Optical Technologies
Gebrekidan, Medhanie
Technische Fakultät
Knipfer, Christian Dr.
Medizinische Fakultät
Stelzle, Florian Prof. Dr. Dr.
Medizinische Fakultät
Will, Stefan Prof. Dr.-Ing.
Lehrstuhl für Technische Thermodynamik


Autor(en) der externen Einrichtung(en)
Leibniz-Institut für Photonische Technologien e.V.


Zitierweisen

APA:
Gebrekidan, M.T., Knipfer, C., Stelzle, F., Popp, J., Will, S., & Bräuer, A. (2016). A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference. Journal of Raman Spectroscopy, 47(2), 198-209. https://dx.doi.org/10.1002/jrs.4775

MLA:
Gebrekidan, Medhanie Tesfay, et al. "A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference." Journal of Raman Spectroscopy 47.2 (2016): 198-209.

BibTeX: 

Zuletzt aktualisiert 2018-03-07 um 01:23