Bioactive Electrospun Fibers of Poly(glycerol sebacate) and Poly(ε-caprolactone) for Cardiac Patch Application

Rai R, Tallawi M, Frati C, Falco A, Gervasi A, Quaini F, Roether J, Hochburger T, Schubert DW, Seik L, Barbani N, Lazzeri L, Rosellini E, Boccaccini AR (2015)

Publication Language: English

Publication Type: Journal article, Review article

Publication year: 2015

Journal

Publisher: John Wiley and Sons Ltd

Book Volume: 13

Pages Range: 2012-2025

Journal Issue: 4

DOI: 10.1002/adhm.201500154

Abstract

Scaffolds for cardiac patch application must meet stringent requirements such as biocompatibility, biodegradability, and facilitate vascularization in the engineered tissue. Here, a bioactive, biocompatible, and biodegradable electrospun scaffold of poly(glycerol sebacate)–poly(ε-caprolactone) (PGS–PCL) is proposed as a potential scaffold for cardiac patch application. The fibers are smooth bead free with average diameter = 0.8 ± 0.3 μm, mean pore size = 2.2 ± 1.2 μm, porosity = 62 ± 4%, and permeability higher than that of control biological tissue. For the first time, bioactive PGS–PCL fibers functionalized with vascular endothelial growth factor (VEGF) are developed, the approach used being chemical modification of the PGS–PCL fibers followed by subsequent binding of VEGF via amide bonding. The approach results in uniform immobilization of VEGF on the fibers; the concentrations are 1.0 μg cm⁻² for the PGS–PCL (H) and 0.60 μg cm⁻² for the PGS–PCL (L) samples. The bioactive scaffold supports the attachment and growth of seeded myogenic and vasculogenic cell lines. In fact, rat aortic endothelial cells also display angiogenic features indicating potential for the formation of vascular tree in the scaffold. These results therefore demonstrate the prospects of VEGF-functionalized PGS–PCL fibrous scaffold as promising matrix for cardiac patch application.

Authors with CRIS profile

Involved external institutions

University of Parma / Università degli Studi di Parma Italy (IT) Ibt – immunological and biochemical testsystems GmbH Germany (DE)

How to cite

APA:

Rai, R., Tallawi, M., Frati, C., Falco, A., Gervasi, A., Quaini, F.,... Boccaccini, A.R. (2015). Bioactive Electrospun Fibers of Poly(glycerol sebacate) and Poly(ε-caprolactone) for Cardiac Patch Application. Advanced Healthcare Materials, 13(4), 2012-2025. https://doi.org/10.1002/adhm.201500154

MLA:

Rai, Ranjana, et al. "Bioactive Electrospun Fibers of Poly(glycerol sebacate) and Poly(ε-caprolactone) for Cardiac Patch Application." Advanced Healthcare Materials 13.4 (2015): 2012-2025.

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