Helicobacter pylori Employs a Unique Basolateral Type IV Secretion Mechanism for CagA Delivery
Beitrag in einer Fachzeitschrift
Details zur Publikation
Autor(en): Tegtmeyer N, Wessler S, Necchi V, Rohde M, Harrer A, Rau T, Asche CI, Böhm M, Loessner H, Figueiredo C, Naumann M, Palmisano R, Solcia E, Ricci V, Backert S
Zeitschrift: → Cell Host & Microbe |
Jahr der Veröffentlichung: 2017
Band: 22
Heftnummer: 4
Seitenbereich: 552-560.e5
ISSN: 1931-3128
Abstract
The Helicobacter pylori (Hp) type IV secretion system (T4SS) forms needle-like pili, whose binding to the integrin-?1 receptor results in injection of the CagA oncoprotein. However, the apical surface of epithelial cells is exposed to Hp, whereas integrins are basolateral receptors. Hence, the mechanism of CagA delivery into polarized gastric epithelial cells remains enigmatic. Here, we demonstrate that T4SS pilus formation during infection of polarized cells occurs predominantly at basolateral membranes, and not at apical sites. Hp accomplishes this by secreting another bacterial protein, the serine protease HtrA, which opens cell-to-cell junctions through cleaving epithelial junctional proteins including occludin, claudin-8, and E-cadherin. Using a genetic system expressing a peptide inhibitor, we demonstrate that HtrA activity is necessary for paracellular transmigration of Hp across polarized cell monolayers to reach basolateral membranes and inject CagA. The contribution of this unique signaling cascade to Hp pathogenesis is discussed.
FAU-Autoren / FAU-Herausgeber
| | | Lehrstuhl für Mikrobiologie |
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| Backert, Steffen Prof. Dr. |
| | Lehrstuhl für Mikrobiologie |
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| | | Lehrstuhl für Mikrobiologie |
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| | | Lehrstuhl für Mikrobiologie |
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| | | Optical Imaging Center Erlangen (OICE) |
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Autor(en) der externen Einrichtung(en)
→ Helmholtz-Zentrum für Infektionsforschung (HZI) |
→ Otto-von-Guericke-Universität Magdeburg |
→ Paul Ehrlich Institute / Paul-Ehrlich-Institut – Bundesinstitut für Impfstoffe und biomedizinische Arzneimittel (PEI) |
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→ Università degli Studi di Pavia |
→ Universität Salzburg (Paris Lodron Universität Salzburg) |
Zitierweisen
APA: | Tegtmeyer, N., Wessler, S., Necchi, V., Rohde, M., Harrer, A., Rau, T.,... Backert, S. (2017). Helicobacter pylori Employs a Unique Basolateral Type IV Secretion Mechanism for CagA Delivery. Cell Host & Microbe, 22(4), 552-560.e5. https://dx.doi.org/10.1016/j.chom.2017.09.005 |
MLA: | Tegtmeyer, Nicole, et al. "Helicobacter pylori Employs a Unique Basolateral Type IV Secretion Mechanism for CagA Delivery." Cell Host & Microbe 22.4 (2017): 552-560.e5. |