Localization of Tubulin and a Centrin-Homologue in Vegetative Cells and Developing Gametangia of Ectocarpus siliculosus (Dillw.) Lyngb. (Phaeophyceae, Ectocarpales) - A combined immunofluorescence and confocal laser scanning microscope study

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Katsaros C, Kreimer G, Melkonian M
Zeitschrift: Botanica Acta : Berichte der Deutschen Botanischen Gesellschaft
Verlag: GEORG THIEME VERLAG
Jahr der Veröffentlichung: 1991
Band: 104
Heftnummer: 2
Seitenbereich: 87-92
ISSN: 0932-8629


Abstract


The distribution of tubulin and centrin in vegetative cells and during gametogenesis of Ectocarpus siliculosus was studied by immunofluorescence. In interphase cells bundles of microtubules are focused on the centriolar region near the nuclear surface. Some of the bundles ensheath the nucleus while others traverse the cytoplasm in various directions, sometimes reaching the cell cortex. Evaluation of serial optical sections by confocal laser scanning microscopy (CLSM) revealed that the perinuclear and "cytoplasmic" microtubule bundles presumably constitute a single complex. In interphase cells centrin is localized as a single bright spot in the centriolar region. In dividing cells duplication and separation of the microtubular complex and the centrin spot takes place. In post-mitotic cells with two nuclei, the centrioles are located at opposite cell poles, short microtubule bundles emanate from them and partially encompass the nucleus. During gametogenesis a gradual transformation of the vegetative cytoskeleton to the gametic flagellar apparatus occurs.




Autor(en) der externen Einrichtung(en)
Universität zu Köln
Εθνικό Μετσόβιο Πολυτεχνείο / National Technical University of Athens


Zitierweisen

APA:
Katsaros, C., Kreimer, G., & Melkonian, M. (1991). Localization of Tubulin and a Centrin-Homologue in Vegetative Cells and Developing Gametangia of Ectocarpus siliculosus (Dillw.) Lyngb. (Phaeophyceae, Ectocarpales) - A combined immunofluorescence and confocal laser scanning microscope study. Botanica Acta : Berichte der Deutschen Botanischen Gesellschaft, 104(2), 87-92. https://dx.doi.org/10.1111/j.1438-8677.1991.tb00201.x

MLA:
Katsaros, Chr, Georg Kreimer, and Michael Melkonian. "Localization of Tubulin and a Centrin-Homologue in Vegetative Cells and Developing Gametangia of Ectocarpus siliculosus (Dillw.) Lyngb. (Phaeophyceae, Ectocarpales) - A combined immunofluorescence and confocal laser scanning microscope study." Botanica Acta : Berichte der Deutschen Botanischen Gesellschaft 104.2 (1991): 87-92.

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