Localization of Tubulin and a Centrin-Homologue in Vegetative Cells and Developing Gametangia of Ectocarpus siliculosus (Dillw.) Lyngb. (Phaeophyceae, Ectocarpales) - A combined immunofluorescence and confocal laser scanning microscope study

Katsaros C, Kreimer G, Melkonian M (1991)

Publication Status: Published

Publication Type: Journal article

Publication year: 1991

Journal

Botanica Acta : Berichte der Deutschen Botanischen Gesellschaft Georg Thieme Verlag

Publisher: GEORG THIEME VERLAG

Book Volume: 104

Pages Range: 87-92

Journal Issue: 2

DOI: 10.1111/j.1438-8677.1991.tb00201.x

Abstract

The distribution of tubulin and centrin in vegetative cells and during gametogenesis of Ectocarpus siliculosus was studied by immunofluorescence. In interphase cells bundles of microtubules are focused on the centriolar region near the nuclear surface. Some of the bundles ensheath the nucleus while others traverse the cytoplasm in various directions, sometimes reaching the cell cortex. Evaluation of serial optical sections by confocal laser scanning microscopy (CLSM) revealed that the perinuclear and "cytoplasmic" microtubule bundles presumably constitute a single complex. In interphase cells centrin is localized as a single bright spot in the centriolar region. In dividing cells duplication and separation of the microtubular complex and the centrin spot takes place. In post-mitotic cells with two nuclei, the centrioles are located at opposite cell poles, short microtubule bundles emanate from them and partially encompass the nucleus. During gametogenesis a gradual transformation of the vegetative cytoskeleton to the gametic flagellar apparatus occurs.

Authors with CRIS profile

Georg Kreimer

Involved external institutions

National Technical University of Athens / Εθνικό Μετσόβιο Πολυτεχνείο GR Greece (GR) Universität zu Köln DE Germany (DE)

How to cite

APA:

Katsaros, C., Kreimer, G., & Melkonian, M. (1991). Localization of Tubulin and a Centrin-Homologue in Vegetative Cells and Developing Gametangia of Ectocarpus siliculosus (Dillw.) Lyngb. (Phaeophyceae, Ectocarpales) - A combined immunofluorescence and confocal laser scanning microscope study. Botanica Acta : Berichte der Deutschen Botanischen Gesellschaft, 104(2), 87-92. https://dx.doi.org/10.1111/j.1438-8677.1991.tb00201.x

MLA:

Katsaros, Chr, Georg Kreimer, and Michael Melkonian. "Localization of Tubulin and a Centrin-Homologue in Vegetative Cells and Developing Gametangia of Ectocarpus siliculosus (Dillw.) Lyngb. (Phaeophyceae, Ectocarpales) - A combined immunofluorescence and confocal laser scanning microscope study." Botanica Acta : Berichte der Deutschen Botanischen Gesellschaft 104.2 (1991): 87-92.

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