Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Klahre U, Friederich E, Kost B, Louvard D, Chua NH
Zeitschrift: Plant Physiology
Verlag: American Society of Plant Biologists
Jahr der Veröffentlichung: 2000
Band: 122
Heftnummer: 1
Seitenbereich: 35-47
ISSN: 0032-0889


Abstract


In an attempt to elucidate the biological function of villin-like actin-binding proteins in plants we have cloned several genes encoding Arabidopsis proteins with high homology to animal villin. We found that Arabidopsis contains at least four villin-like genes (AtVLNs) encoding four different VLN isoforms. Two AtVLN isoforms are more closely related to mammalian villin in their primary structure and are also antigenically related, whereas the other two contain significant changes in the C-terminal headpiece domain. RNA and promoter/beta-glucuronidase expression studies demonstrated that AtVLN genes are expressed in all organs, with elevated expression levels in certain types of cells. These results suggest that AtVLNs have less-specialized functions than mammalian villin, which is found only in the microvilli of brush border cells, Immunoblot experiments using a monoclonal antibody against pig villin showed that AtVLNs are widely distributed in a variety of plant tissues. Green fluorescent protein fused to full-length AtVLN and individual AtVLN headpiece domains can bind to both animal and plant actin filaments in vivo.




Autor(en) der externen Einrichtung(en)
Institut Curie
Rockefeller University


Zitierweisen

APA:
Klahre, U., Friederich, E., Kost, B., Louvard, D., & Chua, N.-H. (2000). Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis. Plant Physiology, 122(1), 35-47. https://dx.doi.org/10.1104/pp.122.1.35

MLA:
Klahre, Ulrich, et al. "Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis." Plant Physiology 122.1 (2000): 35-47.

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