Electrophoretic deposition of gentamicin-loaded bioactive glass/chitosan composite coatings for orthopaedic implants

Pishbin F, Mourino V, Flor S, Kreppel S, Salih V, Ryan MP, Boccaccini AR (2014)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2014

Journal

ACS Applied Materials and Interfaces American Chemical Society

Book Volume: 6

Pages Range: 8796-8806

Journal Issue: 11

DOI: 10.1021/am5014166

Abstract

Despite their widespread application, metallic orthopaedic prosthesis failure still occurs because of lack of adequate bone-bonding and the incidence of post-surgery infections. The goal of this research was to develop multifunctional composite chitosan/Bioglass coatings loaded with gentamicin antibiotic as a suitable strategy to improve the surface properties of metallic implants. Electrophoretic deposition (EPD) was applied as a single-step technology to simultaneously deposit the biopolymer, bioactive glass particles, and the antibiotic on stainless steel substrate. The microstructure and composition of the coatings were characterized using SEM/EDX, XRD, FTIR, and TGA/DSC, respectively. The in vitro bioactivity of the coatings was demonstrated by formation of hydroxyapatite after immersion in simulated body fluid (SBF) in a short period of 2 days. High-performance liquid chromatography (HPLC) measurements indicated the release of 40% of the loaded gentamicin in phosphate buffered saline (PBS) within the first 5 days. The developed composite coating supported attachment and proliferation of MG-63 cells up to 10 days. Moreover, disc diffusion test showed improved bactericidal effect of gentamicin-loaded composite coatings against S. aureus compared to control non-gentamicin-loaded coatings. © 2014 American Chemical Society.

Authors with CRIS profile

Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Imperial College London / The Imperial College of Science, Technology and Medicine GB United Kingdom (GB) Universidad de Buenos Aires (UBA) / University of Buenos Aires AR Argentina (AR) University College London (UCL) GB United Kingdom (GB)

How to cite

APA:

Pishbin, F., Mourino, V., Flor, S., Kreppel, S., Salih, V., Ryan, M.P., & Boccaccini, A.R. (2014). Electrophoretic deposition of gentamicin-loaded bioactive glass/chitosan composite coatings for orthopaedic implants. ACS Applied Materials and Interfaces, 6(11), 8796-8806. https://dx.doi.org/10.1021/am5014166

MLA:

Pishbin, Fatemehsadat, et al. "Electrophoretic deposition of gentamicin-loaded bioactive glass/chitosan composite coatings for orthopaedic implants." ACS Applied Materials and Interfaces 6.11 (2014): 8796-8806.

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