Additive manufacturing of cell-loaded alginate enriched with alkaline phosphatase for bone tissue engineering application

Detsch R, Sarker B, Zehnder T, Boccaccini AR, Douglas TE (2014)


Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2014

Journal

Book Volume: 15

Pages Range: 79-87

Journal Issue: 3-4

DOI: 10.1515/bnm-2014-0007

Abstract

Hydrogels are gaining interest as scaffolds for bone tissue regeneration due to ease of incorporation of cells and biological molecules such as enzymes. Mineralization of hydrogels, desirable for bone tissue regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP). Additive manufacturing techniques such as bioplotting enable the layer-by-layer creation of three-dimensional hydrogel scaffolds with highly defined geometry and internal architecture. In this study, we present a novel method to produce macroporous hydrogel scaffolds in combination with cell-loaded capsule-containing struts by 3D bioplotting. This approach enables loading of the capsules and strut phases with different cells and/or bioactive substances and hence makes compartmentalization within a scaffold possible. 3D porous alginate scaffolds enriched with ALP and MG-63 osteoblast-like cells were produced by bioplotting struts of alginate which were loaded with pre-fabricated alginate capsules. Two combinations were compared, namely ALP in the struts and cells in the capsules and vice-versa. Both combinations were cytocompatible for cells and mineralization of scaffolds could be detected in both cases, according to an OsteoImage staining. ALP had no adverse effect on cytocompatibility and enhanced mitochondrial activity.

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How to cite

APA:

Detsch, R., Sarker, B., Zehnder, T., Boccaccini, A.R., & Douglas, T.E. (2014). Additive manufacturing of cell-loaded alginate enriched with alkaline phosphatase for bone tissue engineering application. BioNanoMaterials, 15(3-4), 79-87. https://dx.doi.org/10.1515/bnm-2014-0007

MLA:

Detsch, Rainer, et al. "Additive manufacturing of cell-loaded alginate enriched with alkaline phosphatase for bone tissue engineering application." BioNanoMaterials 15.3-4 (2014): 79-87.

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