Comparative proteomic profiling of the Choline transporter-like1 (CHER1) mutant provides insights into plasmodesmata composition of fully developed Arabidopsis thaliana leaves.

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Kraner M, Müller C, Sonnewald U
Zeitschrift: The Plant journal : for cell and molecular biology
Jahr der Veröffentlichung: 2017
ISSN: 1365-313X


Abstract


In plants, intercellular communication and exchange are highly dependent on cell wall bridging structures between adhering cells, so-called Plasmodesmata (PD). In our previous genetic screen for PD deficient Arabidopsis mutants, we described choline transporter-like 1 (CHER1) being important for PD genesis and maturation. Leaves of cher1 mutant plants have up to 10-times less PD which do not develop to complex structures. Here we utilize the T-DNA insertion mutant cher1-4 and report a deep comparative proteomic workflow for the identification of cell wall embedded PD-associated proteins. Analyzing triplicates of cell wall enriched fractions in depth by fractionation and quantitative high-resolution mass spectrometry, we compared >5000 proteins obtained from fully developed leaves. Comparative data analysis and subsequent filtering generated a list of 61 proteins being significantly more abundant in Col-0. This list was enriched for previously described PD-associated proteins. To validate PD-association of so far uncharacterised proteins, sub-cellular localisation analysis were carried out by confocal laser scanning microscopy (CLSM). This study confirmed PD-association for three out of four selected candidates indicating that the comparative approach indeed allowed identification of so far undescribed PD-associated proteins. Performing comparative cell wall proteomics of Nicotiana benthamiana tissue we observed an increase in abundance of these three selected candidates during sink to source transition. Taken together, our comparative proteomic approach revealed a valuable data set of potential PD-associated proteins, which can be used as resource to unravel the molecular composition of complex PD and to investigate their function in cell-to-cell communication. This article is protected by copyright. All rights reserved.



FAU-Autoren / FAU-Herausgeber

Kraner, Max Dr.
Sonderforschungsbereich 796 (mit integriertem Graduiertenkolleg) Steuerungsmechanismen mikrobieller Effektoren in Wirtszellen
Müller, Carmen
Sonderforschungsbereich 796 (mit integriertem Graduiertenkolleg) Steuerungsmechanismen mikrobieller Effektoren in Wirtszellen
Sonnewald, Uwe Prof. Dr.
Lehrstuhl für Biochemie


Zitierweisen

APA:
Kraner, M., Müller, C., & Sonnewald, U. (2017). Comparative proteomic profiling of the Choline transporter-like1 (CHER1) mutant provides insights into plasmodesmata composition of fully developed Arabidopsis thaliana leaves. The Plant journal : for cell and molecular biology. https://dx.doi.org/10.1111/tpj.13702

MLA:
Kraner, Max, Carmen Müller, and Uwe Sonnewald. "Comparative proteomic profiling of the Choline transporter-like1 (CHER1) mutant provides insights into plasmodesmata composition of fully developed Arabidopsis thaliana leaves." The Plant journal : for cell and molecular biology (2017).

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Zuletzt aktualisiert 2018-10-08 um 21:26