Cytocompatibility, bioactivity and mechanical strength of calcium phosphate cement reinforced with multi-walled carbon nanotubes and bovine serum albumin
Gholami F, Zein SHS, Gerhardt LC, Low KL, Tan SH, McPhail DS, Grover LM, Boccaccini AR (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Publisher: Elsevier
Book Volume: 39
Pages Range: 4975-4983
Journal Issue: 5
DOI: 10.1016/j.ceramint.2012.11.094
Abstract
This paper reports on the in vitro cytotoxicity, bioactivity behaviour and mechanical properties of novel injectable calcium phosphate cement filled with hydroxylated multi-walled carbon nanotubes and bovine serum albumin (CPC/MWCNT-OH/BSA). To predict the in vitro bioactivity of the calcium phosphate composites, we investigated apatite formation on CPC/MWCNT-OH/BSA composites after soaking in simulated body fluid (SBF) for up to 28 days. Compressive strength tests, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and cell culture experiments with human CCD-18Co fibroblasts cell lines were performed to evaluate the effect of SBF pre-treatment on the mechanical, structural and biological properties of the CPC/MWCNT-OH/BSA composites. Although apatite formation increased significantly with SBF immersion period, the results showed that all soaked CPC/MWCNT-OH/BSA composites exhibited up to 2.5 times lower compressive strength (13-20 MPa), which were however higher than values reported for the strength of trabecular bone (2-12 MPa). Cell culture experiments showed that low concentrations (6.25 and 12.5 μg/ml) of bio-mineralised CPC/MWCNT-OH/BSA composites led to cell proliferative rather than cytotoxic effects on fibroblasts, evidenced by high cell viabilities (104-113%). The novel CPC/MWCNT-OH/BSA composites presented in this study showed favourable cytocompatible and bioactive behaviour along with high compressive strength (13-32 MPa) and are therefore considered as an attractive bone filling material. © 2012 Elsevier Ltd and Techna Group S.r.l.
Authors with CRIS profile
Involved external institutions
University of Birmingham
United Kingdom (GB)
University of Science Malaysia (USM) / Universiti Sains Malaysia
Malaysia (MY)
Imperial College London / The Imperial College of Science, Technology and Medicine
United Kingdom (GB)
United Kingdom (GB)
University of Science Malaysia (USM) / Universiti Sains Malaysia
Malaysia (MY)
Imperial College London / The Imperial College of Science, Technology and Medicine
United Kingdom (GB)
How to cite
APA:
Gholami, F., Zein, S.H.S., Gerhardt, L.C., Low, K.L., Tan, S.H., McPhail, D.S.,... Boccaccini, A.R. (2013). Cytocompatibility, bioactivity and mechanical strength of calcium phosphate cement reinforced with multi-walled carbon nanotubes and bovine serum albumin. Ceramics International, 39(5), 4975-4983. https://dx.doi.org/10.1016/j.ceramint.2012.11.094
MLA:
Gholami, Fatemeh, et al. "Cytocompatibility, bioactivity and mechanical strength of calcium phosphate cement reinforced with multi-walled carbon nanotubes and bovine serum albumin." Ceramics International 39.5 (2013): 4975-4983.
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