Velocity distributions of single F-actin trajectories from a fluorescence image series using trajectory reconstruction and optical flow mapping.

von Wegner F, Ober T, Weber C, Schürmann S, Winter R, Friedrich O, Fink R, Vogel M (2008)

Publication Status: Published

Publication Type: Journal article

Publication year: 2008

Journal

Journal of Biomedical Optics Society of Photo-optical Instrumentation Engineers (SPIE)

Publisher: Society of Photo-optical Instrumentation Engineers (SPIE)

Book Volume: 13

Pages Range: 054018

Journal Issue: 5

DOI: 10.1117/1.2982525

Abstract

We present an approach for the computation of single-object velocity statistics in a noisy fluorescence image series. The algorithm is applied to molecular imaging data from an in vitro actin-myosin motility assay. We compare the relative efficiency of wavelet and curvelet transform denoising in terms of noise reduction and object restoration. It is shown that while both algorithms reduce background noise efficiently, curvelet denoising restores the curved edges of actin filaments more reliably. Noncrossing spatiotemporal actin trajectories are unambiguously identified using a novel segmentation scheme that locally combines the information of 2-D and 3-D segmentation. Finally, the optical flow vector field for the image sequence is computed via the 3-D structure tensor and mapped to the segmented trajectories. Using single-trajectory statistics, the global velocity distribution extracted from an image sequence is decomposed into the contributions of individual trajectories. The technique is further used to analyze the distribution of the x and y components of the velocity vectors separately, and it is shown that directed actin motion is found in myosin extracts from single skeletal muscle fibers. The presented approach may prove helpful to identify actin filament subpopulations and to analyze actin-myosin interaction kinetics under biochemical regulation.

Authors with CRIS profile

Sebastian Schürmann Lehrstuhl für Medizinische Biotechnologie Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie

Involved external institutions

Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Goethe-Universität Frankfurt am Main DE Germany (DE)

How to cite

APA:

von Wegner, F., Ober, T., Weber, C., Schürmann, S., Winter, R., Friedrich, O.,... Vogel, M. (2008). Velocity distributions of single F-actin trajectories from a fluorescence image series using trajectory reconstruction and optical flow mapping. Journal of Biomedical Optics, 13(5), 054018. https://doi.org/10.1117/1.2982525

MLA:

von Wegner, Frederic, et al. "Velocity distributions of single F-actin trajectories from a fluorescence image series using trajectory reconstruction and optical flow mapping." Journal of Biomedical Optics 13.5 (2008): 054018.

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